The DNAL1 Knockout NCI-H1299 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population derived from the NCI-H1299 human lung carcinoma cell line, designed to disrupt expression of the DNAL1 gene. As a heterogeneous pool, this product avoids clonal selection artifacts and provides a versatile loss-of-function model for investigating DNAL1-dependent biological processes.
The host NCI-H1299 line was established from a lymph node metastasis of a lung adenocarcinoma and is widely employed as a model for non-small cell lung cancer (NSCLC) with high metastatic potential. This cell line is p53-null and KRAS wild-type, offering a specific genetic background for studying tumor progression and signaling mechanisms independent of p53-mediated regulation.
DNAL1 encodes an axonemal dynein light chain essential for outer dynein arm assembly and ciliary motility. It is transcriptionally regulated by FOXJ1 and RFX3, and functionally interacts with dynein heavy chains DNAH5 and DNAH11. Disruption of DNAL1 impairs ciliogenesis, thereby attenuating cilia-dependent Hedgehog signaling, as evidenced by reduced GLI1 and GLI2 transcriptional activity. In parallel, DNAL1 influences cell cycle progression through modulation of Cyclin D1 and CDK4, linking ciliary function to proliferative control.
In the NCI-H1299 background, DNAL1 knockout is anticipated to compromise ciliary function, potentially altering Hedgehog-driven transcriptional programs and cell cycle regulation. Given the metastatic origin of this line, the loss of DNAL1 may also affect invasive and migratory properties, offering a physiologically relevant system to dissect the interplay between ciliary dynamics and NSCLC malignancy.
This polyclonal knockout pool supports a broad range of experimental applications, including the study of cilia-dependent signaling pathways in NSCLC, functional analysis of DNAL1 in cell proliferation and metastasis, disease modeling of primary ciliary dyskinesia, and evaluation of drug responses. Researchers can validate knockout effects via Western blotting for DNAL1 and ciliary markers, RT?qPCR for GLI1 and CCND1, immunofluorescence staining of acetylated tubulin and ???tubulin, cell proliferation and migration assays, flow cytometry for cell cycle analysis, and transcriptome profiling by RNA?seq. For further technical details or custom solutions, please contact Ascent Research.
