The DNPH1 Knockout AGS Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from the AGS human gastric adenocarcinoma epithelial cell line. This model is designed for loss-of-function studies of the DNPH1 gene, which encodes an enzyme that hydrolyzes 2′-deoxynucleoside 5′-monophosphates. The polyclonal population provides a heterogeneous mixture of edited alleles, enabling researchers to investigate DNPH1 function in a physiologically relevant context without the isolation of single-cell clones. This product is an ideal tool for studying nucleotide metabolism and its link to cellular proliferation in gastric cancer.
The AGS cell line is a well-established adherent epithelial model derived from a human gastric adenocarcinoma. It is widely used in gastric cancer research, including studies of Helicobacter pylori infection, oncogenic signaling, and drug response. AGS cells retain key features of gastric epithelium, making them a relevant system for dissecting molecular pathways involved in gastric carcinogenesis. The integration of DNPH1 knockout into this background allows the examination of gene function in a disease-relevant cellular environment.
DNPH1 functions as a nucleotidase that catalyzes the hydrolysis of 2′-deoxynucleoside 5′-monophosphates to deoxyribose 5-phosphate and free nucleobases, playing a critical role in nucleotide salvage and the regulation of intracellular nucleotide pools. The expression of DNPH1 is directly regulated by the c-Myc transcription factor, linking its activity to growth factor signaling and cellular proliferation. Downstream, DNPH1 activity influences the availability of deoxyribose 5-phosphate and nucleobases, which are important for maintaining nucleotide homeostasis. Through these interactions, DNPH1 modulates metabolic flux within nucleotide metabolism pathways, affecting DNA synthesis and cell cycle progression.
In the context of gastric adenocarcinoma, DNPH1 is implicated in c-Myc-dependent proliferation, a hallmark of many cancers. Since AGS cells are often used to model gastric cancer with c-Myc dysregulation, the DNPH1 knockout polyclonal population provides a valuable tool for dissecting the contribution of nucleotide salvage to tumor cell growth. Additionally, given the role of Helicobacter pylori in gastric disease, this model can be employed to study host-pathogen interactions and the impact of nucleotide metabolism on infection outcomes. The knockout may reveal vulnerabilities that can be targeted therapeutically.
Researchers can employ a variety of assays with these DNPH1 knockout polyclonal cells, including Western blot and RT-qPCR for expression analysis, genomic PCR and sequencing for genotype verification, and nucleotide pool quantification. Functional studies may involve proliferation, apoptosis, and cell cycle analyses, as well as drug sensitivity testing to evaluate the role of nucleotide salvage in chemoresistance. The model is also suitable for Helicobacter pylori infection assays to investigate pathogen-induced alterations in host metabolism. For further information or customized solutions, please contact Ascent Research.
