The DNPH1 Knockout Ca Ski Polyclonal Cells product consists of a heterogeneous population of Ca Ski human cervical carcinoma cells that have undergone CRISPR/Cas9-mediated gene disruption targeting the DNPH1 locus, generating a pooled loss-of-function model. This polyclonal knockout cell population is designed to enable functional investigation of DNPH1 without requiring single-cell clone isolation, providing a representative spectrum of edited alleles for population-based studies.
The host cell line, Ca Ski, is an adherent epithelial line derived from a metastatic cervical epidermoid carcinoma and harbors integrated human papillomavirus type 16 (HPV16) sequences. As a well-established model of HPV-driven cervical cancer, Ca Ski cells recapitulate key features of the disease, including deregulated cell cycle control and oncogene-driven metabolism. This background is particularly relevant for studying the c-Myc transcriptional network and nucleotide metabolism in the context of viral oncogenesis.
DNPH1 encodes a nucleoside diphosphate phosphatase that hydrolyzes diphosphate nucleotides such as GDP and UDP, thereby modulating nucleotide pools important for RNA processing and cellular proliferation. Mechanistically, DNPH1 is transcriptionally induced by the MYC oncoprotein and acts as an effector of c-Myc signaling, linking growth factor-mediated signals to nucleotide salvage and pyrimidine metabolism. The enzyme interacts directly with its nucleotide substrates and is positioned upstream of RNA processing machinery, and its activity is integrated into a pathway containing MYC and other nucleotide metabolism enzymes.
In the Ca Ski cell context, disruption of DNPH1 offers a powerful system to dissect the role of MYC-driven nucleotide metabolism in cervical cancer progression. Because HPV16-positive cells frequently exhibit elevated MYC activity, these polyclonal knockout cells allow researchers to examine how loss of DNPH1 affects nucleotide pool homeostasis, RNA processing fidelity, and cell cycle dynamics. The model is uniquely suited to explore the interplay between viral oncoproteins, transcription factor networks, and metabolic reprogramming.
Researchers can employ these polyclonal knockout cells in a variety of assays, including western blotting and RT-qPCR for confirmation of target disruption, cell proliferation and viability assays under different nucleotide conditions, high-performance liquid chromatography or mass spectrometry-based nucleotide pool measurements, RNA sequencing to evaluate transcriptome-wide processing changes, and flow cytometry to assess cell cycle distribution. These applications support investigation of DNPH1 as a potential therapeutic target in HPV-associated cancers and as a mediator of c-Myc-dependent proliferation. For further details on product specifications and pricing, please contact Ascent Research.
