The DNPH1 Knockout Raji Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji B lymphocyte line. This heterogeneous pool carries diverse loss-of-function mutations at the DNPH1 locus, eliminating the need for single-cell cloning and avoiding clonal bias. The polyclonal format is ideal for functional genomics, drug screening, and metabolic profiling experiments where a population-averaged phenotype is desirable.
The parental Raji cell line, an EBV-positive Burkitt??s lymphoma B lymphocyte model, is widely used to study B cell receptor signaling and MYC-driven transcriptional programs. Raji cells grow in suspension and exhibit constitutive activation of survival pathways characteristic of aggressive B cell malignancies, providing a physiologically relevant host for investigating nucleotide metabolism and lymphoma biology.
DNPH1 encodes a nucleoside monophosphate N-glycosidase that hydrolyzes dCMP, dUMP, and dTMP into free nucleobases and 2-deoxyribose 5-phosphate. Transcriptionally regulated by MYC and E2F, DNPH1 acts downstream of these oncogenic transcription factors to modulate deoxynucleoside triphosphate pools, which serve as substrates for DNA polymerases during DNA replication. Through its control of nucleotide homeostasis, DNPH1 links proliferation signals to DNA synthesis capacity.
In Raji cells, which overexpress MYC and have a high demand for nucleotide biosynthesis, DNPH1 disruption likely perturbs dNMP catabolism, leading to altered nucleotide pools, replication stress, and enhanced sensitivity to nucleoside analog drugs. This polyclonal knockout model enables dissection of nucleotide salvage and catabolism pathways in a B cell lymphoma context and facilitates exploration of DNPH1 as a therapeutic vulnerability in MYC-driven tumors.
Typical applications include proliferation assays, drug sensitivity testing with nucleoside analogs, nucleotide pool analysis, and apoptosis profiling. Relevant assays include Western blotting, RT-qPCR, DNA sequencing for editing confirmation, flow cytometry for cell cycle profiling, cell proliferation assays, nucleotide pool measurement, and apoptosis detection. This polyclonal format supports robust pooled analyses, making it suitable for target validation and functional screening. For additional information or custom gene-editing requests, please contact Ascent Research.
