DNTTIP1 Knockout HAP1 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human HAP1 cell line, carrying a targeted disruption of the DNTTIP1 locus. This product provides a loss-of-function model for investigating the biological roles of DNTTIP1, a component of the little elongation complex involved in RNA polymerase II-mediated transcription of small nuclear RNA genes. The polyclonal format ensures a heterogeneous mixture of knockout alleles, enabling robust functional studies without clonal artifacts.
The host HAP1 cell line is a human near-haploid chronic myeloid leukemia-derived line originally established from the KBM-7 cell line. Its haploid nature facilitates efficient gene disruption and straightforward genotype-phenotype correlation, making it a widely adopted host for CRISPR-based knockout screens. HAP1 cells retain key signaling and transcriptional networks, providing a physiologically relevant context for studying DNTTIP1 in a cancer cell background.
DNTTIP1 functions within the little elongation complex, which promotes transcription elongation of snRNAs (U1, U2, U4, U5) by RNA polymerase II. It interacts directly with terminal deoxynucleotidyltransferase (TdT) and forms complexes with ICE1, ICE2, and NELFB. Upstream, DNTTIP1 is regulated by MYC and acts downstream of RNA polymerase II recruitment. Thus, disruption of DNTTIP1 is anticipated to impair snRNA biogenesis and potentially affect DNA repair processes linked to TdT.
In the HAP1 model, DNTTIP1 knockout offers a unique system to dissect its contributions to neurodevelopmental disorders and cancer, where dysregulated transcription is implicated. The near-haploid background enhances the sensitivity of functional genomic screens, enabling detection of subtle phenotypes in snRNA expression and cell growth. This cell population is ideal for studying the interplay between transcription elongation and genome stability in a leukemia-derived context.
Researchers can employ these knockout cells for a variety of applications, including RT-qPCR and RNA-seq to quantify snRNA levels, ChIP-qPCR to assess RNA polymerase II occupancy at snRNA gene promoters, and western blotting for protein expression analysis. They are suitable for functional genomics screening and mechanistic studies of neurodevelopmental and oncogenic pathways. For additional details or custom inquiries, please contact Ascent Research.
