The DNTTIP1 Knockout HGC-27 Polyclonal Cells product consists of a CRISPR/Cas9-edited polyclonal population of HGC-27 human gastric carcinoma cells with targeted disruption of the DNTTIP1 gene. This heterogeneous pool of gene-edited cells provides a loss-of-function model for studying DNTTIP1-dependent processes without clonal selection biases, making it suitable for experiments where a polyclonal representation of knockout effects is desired.
The HGC-27 host cell line was established from a metastatic lymph node of a female patient with gastric adenocarcinoma and exhibits an epithelial-like, adherent morphology. As a widely used gastric cancer model, HGC-27 cells retain relevant oncogenic features and are valuable for dissecting molecular mechanisms of gastric carcinogenesis, metastasis, and therapeutic response.
DNTTIP1 (TdIF1) is a nucleolar protein that functions as a transcriptional repressor of ribosomal RNA (rRNA) synthesis. It binds to rDNA promoters and inhibits 45S pre-rRNA transcription by competing with the upstream binding factor (UBF), thereby downregulating RNA Polymerase I activity and ribosome biogenesis. DNTTIP1 interacts with deoxynucleotidyltransferase (DNTT), histone deacetylase 1 (HDAC1), and nucleophosmin (NPM1), linking it to chromatin remodeling and nucleolar dynamics. Its activity is integrated into nucleolar stress responses and cell cycle regulation, placing DNTTIP1 at a critical intersection of growth signaling and translational control.
In the HGC-27 gastric cancer background, DNTTIP1 knockout offers a physiologically relevant system to explore its contribution to malignant phenotypes. Given the frequent dysregulation of nucleolar function and ribosome biogenesis in gastric cancer, this polyclonal knockout model enables assessment of DNTTIP1-dependent effects on cell proliferation, survival, and nucleolar integrity within a metastatic lymph node-derived context, providing insights into advanced disease biology.
This knockout product supports a broad range of research applications. Western blotting confirms DNTTIP1 depletion, while RT-qPCR and ChIP-qPCR enable quantification of rRNA transcripts and rDNA occupancy. Functional assays include MTT or BrdU proliferation studies, apoptosis detection, cell cycle analysis by flow cytometry, and immunofluorescence for nucleolar markers. Transcriptome profiling via RNA-seq can reveal global consequences of DNTTIP1 loss. The polyclonal format is well-suited for pooled functional genomics screens or for validating inhibitors of ribosome biogenesis. For further details or custom requests, please contact Ascent Research.
