DPP7 Knockout AGS Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the AGS human gastric adenocarcinoma cell line. This product provides a genetically disrupted DPP7 locus, enabling loss-of-function studies in a gastric cancer background. The polyclonal format offers a heterogeneous pool of edited cells, suitable for experiments where clonal variation is not a primary concern. It serves as a ready-to-use knockout model for investigating dipeptidyl peptidase 7.
The AGS cell line originates from a human gastric adenocarcinoma and exhibits epithelial morphology. Widely used in gastric cancer biology, it supports studies on tumor cell proliferation, apoptosis, and epithelial barrier function. AGS cells are also employed to examine host-pathogen interactions, particularly Helicobacter pylori infection. This well-characterized line provides a relevant and reproducible platform for functional genomics in gastric malignancy.
DPP7 encodes a lysosomal serine aminodipeptidase that removes N-terminal dipeptides from protein substrates, contributing to protein turnover within the lysosome. Its activity is regulated by nutrient and oxidative stress, p53 signaling, and TFEB. Downstream, DPP7 processing influences neuropeptides like NPY and substance P, and modulates apoptosis regulators including caspase substrates. It interacts with LAMP1 and works with cathepsins and lysosomal hydrolases, linking lysosomal proteolysis to mitochondrial homeostasis and apoptotic pathways involving BCL2 family proteins and caspases.
Disruption of DPP7 in AGS cells impairs lysosomal dipeptidyl peptidase activity, altering protein catabolism and apoptotic signaling. This can affect gastric cancer cell growth, quiescence-proliferation balance, and stress responses. The knockout model enables dissection of how DPP7-dependent cleavage impacts survival and death decisions, as well as lysosome-mitochondria crosstalk. Using these polyclonal knockout cells, researchers can investigate DPP7??s role in gastric cancer progression and its potential to modulate drug sensitivity.
These cells are suited for functional assays including apoptosis monitoring by western blotting for cleaved caspases, gene expression analysis by RT-qPCR, and cell cycle assessment by flow cytometry. Lysosomal enzyme activity, mitochondrial function, and proliferation assays provide insight into metabolic alterations. They are also applicable in drug sensitivity screens and H. pylori infection studies. For further information or to discuss your experimental needs, please contact Ascent Research.
