The DPP7 Knockout HGC-27 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the HGC-27 human gastric carcinoma cell line. This heterogeneous pool carries targeted gene disruptions in dipeptidyl peptidase 7 (DPP7), enabling functional studies without clonal selection bias. The polyclonal format is ideal for assessing loss-of-function effects on protein turnover, antigen processing, and apoptosis regulation in a gastric cancer context.
The HGC-27 cell line, established from a lymph node metastasis of a gastric cancer patient, serves as a widely used model for gastric adenocarcinoma. These epithelial cells exhibit rapid proliferation and invasive properties, making them suitable for studying tumor cell biology, drug resistance, and metastasis. This background provides a physiologically relevant system to evaluate DPP7??s contribution to gastric cancer phenotypes.
DPP7 encodes a lysosomal serine protease that cleaves N-terminal dipeptides from proline-containing peptides, participating in protein degradation and regulating bioactive peptides such as neuropeptides and hormones. By trimming antigenic peptides, DPP7 influences MHC class I presentation and immune surveillance. In quiescent cells, DPP7 activity promotes survival by processing pro-apoptotic substrates; its loss may activate apoptotic signaling through mitochondrial pathways. The enzyme likely interacts with other lysosomal proteases and may be regulated by cellular stress and quiescence.
In gastric cancer, DPP7 may sustain tumor cell homeostasis by facilitating protein turnover and suppressing apoptosis. Knocking out DPP7 in HGC-27 cells allows investigation of how loss of this peptidase affects cancer cell fitness, proliferation, and stress responses. This model can help uncover DPP7-dependent vulnerabilities and interactions with oncogenic pathways, providing insights into gastric adenocarcinoma biology and potential therapeutic targets.
Researchers can use these polyclonal knockout cells for MTT proliferation assays, Annexin V apoptosis detection, and Transwell migration/invasion studies. Transcriptomic profiling via RNA-seq and RT-qPCR, along with peptidase activity measurements, facilitate comprehensive phenotyping. This tool supports research into DPP7??s role in cancer cell biology, antigen processing, and stress responses. For further information or custom requests, please contact Ascent Research.
