The DPP8 Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population featuring targeted disruption of the DPP8 gene in the A2780 human ovarian carcinoma cell line. This knockout model eliminates DPP8 enzymatic activity across a heterogeneous cell pool, providing a physiologically relevant system to investigate loss-of-function effects without clonal selection bias. The polyclonal format ensures representation of diverse mutational outcomes, capturing the functional heterogeneity of DPP8 deficiency as it occurs in a population context.
The A2780 cell line is an established human ovarian carcinoma model derived from an untreated patient, exhibiting adherent epithelial morphology. It is widely used in ovarian cancer research to study tumor cell biology, drug responses, and signaling pathways. A2780 cells retain key characteristics of high-grade serous ovarian carcinoma, including relevant oncogenic mutations and signaling dependencies, making them a suitable platform for functional genomics and target validation studies.
DPP8 encodes a cytosolic serine protease with N-terminal dipeptidyl peptidase activity, preferentially cleaving substrates after proline residues. It plays a critical role in regulating immune responses and apoptosis by modulating the NLRP3 inflammasome pathway. DPP8 is activated by upstream signals such as IFN-??, TNF-??, and cellular stress, and functions in a complex with the closely related enzyme DPP9. Through interactions with NLRP3, ASC, and caspase-1, DPP8 suppresses inflammasome assembly and pro-inflammatory cytokine maturation. Downstream targets include chemokines, neuropeptides, and growth factors, as well as the proteolytic processing of pro-IL-1?? by caspase-1. Disruption of DPP8 relieves this suppression, promoting NLRP3 inflammasome activation and subsequent IL-1?? release, thereby connecting innate immune signaling to cell survival and proliferation pathways.
In the context of A2780 ovarian cancer cells, DPP8 knockout creates a powerful tool to dissect the cross-talk between inflammasome activation and tumor cell behavior. Ovarian cancer is characterized by a complex inflammatory microenvironment, where cytokines influence tumor progression, immune evasion, and response to therapy. By abrogating DPP8 function, researchers can study how derepression of NLRP3 and altered processing of proline-containing substrates affect A2780 cell viability, apoptosis, and immunogenicity. This model is particularly relevant for investigating the dual roles of DPP8 in cancer cell-intrinsic pathways and the broader immune context, including potential effects on T-cell signaling and cytokine networks.
Typical applications include mechanistic studies on inflammasome regulation, immune cell activation, and ovarian cancer proliferation, as well as drug target validation and functional genomics screening. Representative assays compatible with this model include Western blotting for DPP8 and NLRP3, ELISA for secreted IL-1??, caspase-1 activity assays, MTT-based cell viability measurements, flow cytometry for apoptosis and cell cycle analysis, and cytokine profiling using multiplexed immunoassays. For detailed product information, lot-specific quality control data, or technical support, please contact Ascent Research.
