The DPPA4 Knockout HAP1 Polyclonal Cells product supplies a polyclonal cell population generated by CRISPR/Cas9-mediated gene disruption of DPPA4 in the HAP1 near-haploid human cell line. This loss-of-function model provides a mixed pool of edited alleles, ensuring a representative knockout phenotype while avoiding the artifacts associated with clonal selection. The polyclonal format is particularly suited for large-scale functional genomics experiments and pooled screening approaches.
HAP1 is a near-haploid, fibroblastoid cell line originally derived from KBM-7 chronic myeloid leukemia cells, characterized by adherent growth and a predominantly haploid karyotype. The near-haploid nature of HAP1 cells greatly facilitates CRISPR-based knockout studies, as a single disruptive editing event is sufficient to abolish gene function, thereby enhancing the efficiency and reliability of genetic screens and mechanistic investigations in cancer and developmental biology.
DPPA4 is a core pluripotency factor that cooperates with OCT4, SOX2, and NANOG to maintain embryonic stem cell self-renewal. It is transcriptionally regulated by OCT4, SOX2, NANOG, and STAT3, and it in turn contributes to the activation of OCT4, NANOG, SOX2, and cell cycle regulators. DPPA4 physically interacts with NANOG, SOX2, OCT4, and SALL4, forming a tightly knit regulatory network. Signaling inputs from LIF/STAT3, Wnt/??-catenin, and TGF-??/SMAD2/3 converge on this network, integrating environmental cues to control pluripotency gene expression.
In the HAP1 context, DPPA4 disruption dismantles the core pluripotency transcriptional network, potentially leading to spontaneous differentiation or loss of self-renewal capacity. The leukemic origin of HAP1 cells provides a unique opportunity to examine pluripotency factor function outside the embryonic stem cell paradigm, offering insights into oncogenic reprogramming. Because DPPA4 is implicated in germ cell tumors and testicular cancer, this knockout model serves as a valuable tool for studying the molecular mechanisms underlying these malignancies.
This product enables a wide range of experimental applications, including gene expression profiling via RNA-seq, protein analysis by Western blotting, and functional studies such as colony formation, differentiation, and alkaline phosphatase activity assays. Immunofluorescence and flow cytometry allow single-cell-level analysis of pluripotency markers. Moreover, the near-haploid background permits high-throughput drug sensitivity screens to identify compounds that selectively target DPPA4-deficient cells. For additional product details, technical support, or custom services, please contact Ascent Research.
