The DPYSL5 Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed for loss-of-function studies of the DPYSL5 gene in human epithelial ovarian cancer cells. This product provides a genetically disrupted polyclonal pool of A2780 cells, offering a heterogeneous knockout model suitable for investigating the functional consequences of DPYSL5 deficiency.
The A2780 cell line is a well-established epithelial ovarian carcinoma model derived from an untreated patient. These cells retain cisplatin sensitivity, making them a valuable system for studying drug response mechanisms. The A2780 background is widely used to explore ovarian cancer biology, including cell migration, invasion, and signaling pathways relevant to metastasis and chemoresistance.
DPYSL5 encodes collapsin response mediator protein 5 (CRMP5), a member of the CRMP family that regulates cytoskeletal dynamics. CRMP5 functions downstream of semaphorin 3A (SEMA3A) signaling, which is transduced by the neuropilin-1/plexin-A receptor complex. Upon activation, CRMP5 undergoes phosphorylation by glycogen synthase kinase 3 beta (GSK3??) and interacts with tubulin heterodimers, actin-binding proteins, and other CRMP family members to modulate microtubule assembly and actin reorganization. This pathway integrates with Rho GTPase signaling to control cell morphology and motility.
In ovarian cancer cells, aberrant cytoskeletal regulation contributes to enhanced migratory and invasive capacities. DPYSL5 disruption in the A2780 background likely perturbs semaphorin-mediated cytoskeletal remodeling, thereby altering cell migration and invasion potential. This model enables dissection of CRMP5-dependent mechanisms that may link axon guidance-like signaling to ovarian cancer progression, providing insights into metastatic behavior.
These polyclonal knockout cells are suitable for a range of functional assays, including wound healing and transwell migration/invasion studies to assess motility, immunofluorescence analysis of F-actin and tubulin organization, and molecular profiling via western blotting, RT-qPCR, or RNA-seq. Phospho-signaling arrays can be employed to monitor pathway alterations. The DPYSL5 Knockout A2780 Polyclonal Cells thus serve as a versatile tool for investigations into cytoskeletal regulation, metastasis research, and neuronal development modeling. For further details or custom inquiries, please contact Ascent Research.
