The DUOX2 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population derived from the near-haploid HAP1 cell line, harboring loss-of-function mutations in the DUOX2 gene. This polyclonal knockout format provides a heterogeneous genetic background that avoids clonal biases and offers a robust model for studying DUOX2-dependent cellular functions. By disrupting DUOX2 via CRISPR/Cas9-mediated gene targeting, these cells enable detailed analysis of hydrogen peroxide production and related signaling pathways in a tractable host system.
HAP1 cells originate from KBM-7 chronic myeloid leukemia cells and possess a near-haploid karyotype, which greatly facilitates gene editing and functional genomics studies. Their haploid genome ensures unambiguous genotype-phenotype correlations and has made them a preferred model for arrayed and pooled genetic screens. This host background is particularly suitable for knockout-based investigations, as single-gene disruption typically yields clear loss-of-function effects without interference from a second allele.
The DUOX2 gene encodes dual oxidase 2, a transmembrane NADPH oxidase that generates extracellular hydrogen peroxide. Its activity requires the maturation factor DUOXA2 and is regulated by intracellular calcium. DUOX2-produced H2O2 serves as a substrate for thyroperoxidase in thyroid hormone biosynthesis and for lactoperoxidase in mucosal innate immunity. Upstream, cytokines such as IL-4 and IL-13 and pathogen-associated molecular patterns induce DUOX2 expression, while downstream targets include NF-??B and protein tyrosine phosphatases, linking DUOX2 to ROS signaling and inflammatory responses. Key pathway components include DUOX2, DUOXA2, TPO, pendrin, NIS, and lactoperoxidase.
In the HAP1 near-haploid context, DUOX2 knockout cells allow direct assessment of the enzyme’s role in generating reactive oxygen species and modulating downstream pathways. The polyclonal nature of the product makes it well-suited for unbiased genetic modifier screens and for examining the interplay between DUOX2-driven oxidative signaling and oncogenic networks in a leukemia-derived lineage. This model enables researchers to dissect DUOX2 functions without the complication of diploid allele redundancy.
These polyclonal knockout cells are intended for a broad spectrum of research applications, including thyroid hormone synthesis studies, ROS signaling analysis, host-pathogen interaction assays, and inflammatory disease modeling. Representative techniques include western blotting, RT-qPCR, immunofluorescence, Amplex Red H2O2 measurement, flow cytometry for ROS detection, and co-immunoprecipitation of DUOX2 protein complexes. For additional product details, please contact Ascent Research.
