The DUS3L Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population targeting the DUS3L gene in HeLa cells. This loss-of-function model is designed to study dual-specificity phosphatase 3-like (DUS3L) function in human epithelial cells. The polyclonal nature provides a heterogeneous knockout background, minimizing clonal artifacts. The product is validated for gene disruption and is ideal for examining DUS3L-dependent MAPK signaling regulation.
HeLa cells are a HPV18-positive cervical adenocarcinoma line with inactivated p53 and Rb due to viral E6 and E7 oncoproteins. This immortalized background supports deregulated proliferation, making it a standard model for cancer signaling research. The epithelial origin and robust growth characteristics ensure consistent experimental performance across a variety of biochemical and cell-based assays.
DUS3L acts as a dual-specificity phosphatase that negatively regulates MAP kinase cascades by dephosphorylating tyrosine and serine/threonine residues on MAPK1 (ERK2), MAPK8 (JNK1), and MAPK14 (p38??). Its phosphatase domain directly interacts with these kinases to dampen signals elicited by growth factors, oxidative, and osmotic stress. Upstream receptor tyrosine kinases such as EGFR activate RAS-RAF-MEK-ERK signaling, while stress stimuli trigger JNK and p38 pathways. DUS3L-mediated attenuation is critical for controlling cell proliferation, differentiation, and stress responses.
Within HeLa cells, HPV-driven constitutive proliferative signaling renders MAPK pathway regulation particularly consequential. Loss of DUS3L likely potentiates ERK, JNK, and p38 activity, providing a tool to investigate phosphatase-dependent modulation of oncogenic and stress responses. This model is valuable for studying cervical adenocarcinoma biology and the broader role of dual-specificity phosphatases in cancer.
Researchers can employ this knockout product in Western blotting for phospho-ERK, phospho-JNK, and phospho-p38 to monitor pathway activation, in cell proliferation assays such as MTT or BrdU, and in apoptosis evaluation via Annexin V staining. RT-qPCR enables quantification of MAPK target genes, while immunofluorescence reveals subcellular kinase distribution. These applications support the elucidation of DUS3L??s regulatory functions in MAPK signaling. For further information, please contact Ascent Research.
