DUSP2 Knockout HAP1 Polyclonal Cells provide a CRISPR/Cas9-edited polyclonal knockout cell population for studying loss-of-function effects of the DUSP2 gene. This product offers a genetically disrupted DUSP2 locus in the HAP1 human cell line, enabling detailed analysis of DUSP2??s role in mitogen-activated protein kinase (MAPK) signaling regulation and immune-related pathways.
HAP1 is a near-haploid, fibroblast-like human cell line derived from the male chronic myeloid leukemia cell line KBM-7. Its adherent growth and haploid genotype simplify genetic studies, as single gene disruptions produce clear phenotypes. This makes HAP1 an ideal host for functional genomics and CRISPR-based knockout screens, including investigations into signaling networks and drug targets.
DUSP2 (also known as PAC1) encodes a dual-specificity phosphatase that negatively regulates MAPK cascades by dephosphorylating phospho-tyrosine and phospho-threonine residues on ERK1/2, JNK1/2, and p38 MAPK. DUSP2 activity is induced by T cell receptor stimulation, PMA/ionomycin, growth factors, and oxidative stress, and is transcriptionally controlled by NFAT and AP-1. By inactivating these MAPKs, DUSP2 attenuates AP-1-mediated transcription, thereby modulating cytokine production and immune cell activation. The broader signaling network includes upstream kinases MEK, MKK4, and MKK7, and downstream transcription factors such as NFAT and AP-1.
In the HAP1 background, DUSP2 knockout enables dissection of its phosphatase activity in a simplified, non-immune cellular context where baseline MAPK signaling components are conserved. Although HAP1 is fibroblast-like, DUSP2??s regulatory function on MAPK modules is shared across cell types, making this model valuable for studying fundamental signal transduction mechanisms and feedback regulation. The haploid nature ensures that even modest effects on pathway kinetics can be unambiguously attributed to DUSP2 loss.
This polyclonal knockout product is suited for functional genomics, signaling studies, and drug target validation. Researchers can employ western blotting for phospho-ERK, JNK, and p38 to assess MAPK activation, RT-qPCR or RNA-seq for transcriptional profiling, and luciferase reporter assays for AP-1/NFAT activity. Additionally, cytokine ELISA, apoptosis and migration assays, and phospho-signaling analysis can be performed to investigate DUSP2??s role in immune and inflammatory responses. For technical specifications and ordering details, please contact Ascent Research.
