DUSP3 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the DUSP3 gene in the human HEK293T embryonic kidney epithelial cell line. The polyclonal format provides a heterogeneous pool of edited cells, offering a robust loss-of-function model for functional genomics studies without clonal selection. This knockout model enables investigation of DUSP3-dependent signaling regulation and cellular phenotypes in a high-transfectability background.
HEK293T cells are derived from HEK293 cells and stably express the SV40 large T antigen, facilitating episomal replication of plasmids bearing the SV40 origin. This feature, combined with their epithelial morphology and high transfection efficiency, makes them a widely used platform for transient protein expression, viral packaging, and gene-editing applications. The adherent growth and rapid proliferation further support scalable cell-based assays.
DUSP3, also known as VHR, is a dual-specificity phosphatase that dephosphorylates phosphotyrosine and phosphothreonine/serine residues, with key substrates including MAPK1/3 (ERK1/2), MAPK8/9 (JNK1/2), and MAPK14 (p38??). By reversing the TXY motif phosphorylation, DUSP3 negatively regulates MAP kinase cascades. Its activity is modulated by upstream signals such as EGFR, TNF-??, IL-1??, and oxidative stress. Downstream, DUSP3 influences STAT3, FAK, and transcriptional programs controlling cell proliferation and survival. Thus, DUSP3 sits at a regulatory hub integrating growth factor, inflammatory, and stress cues.
In HEK293T cells, DUSP3 disruption leads to hyperactivation of ERK, JNK, and p38 pathways, potentially altering proliferative responses, apoptosis thresholds, and stress resistance. This cellular context is particularly relevant for studying oncogenic signaling, as HEK293T cells exhibit transformed characteristics. The polyclonal knockout pool avoids compensation biases associated with single-cell clones and better mimics heterogeneous tissue responses, providing a physiologically relevant model for signaling perturbations.
This knockout product is suited for a broad range of experimental applications, including phospho-signaling profiling by western blotting or phospho-specific flow cytometry, RT-qPCR analysis of immediate early genes, proliferation and cell cycle assays, apoptosis studies, migration/invasion assays, and drug sensitivity screens. Researchers can utilize these cells to screen protein phosphatase inhibitors, investigate DNA damage response mechanisms, or map DUSP3-dependent signaling interactions. For additional information or custom requirements, please contact Ascent Research.
