The DVL2 Knockout HEK293T Polyclonal Cells comprise a CRISPR/Cas9-edited polyclonal knockout population of the HEK293T human cell line, offering a loss-of-function model for the DVL2 gene, which encodes the Wnt signaling scaffold dishevelled segment polarity protein 2. The polyclonal mixture contains cells with diverse DVL2 disruptions, suitable for bulk functional assays without clonal selection.
The HEK293T host cell line is a derivative of human embryonic kidney 293 cells, stably expressing SV40 large T-antigen for high-level plasmid amplification and protein expression. Widely used for transfection-based experiments, these adherent epithelial cells provide a robust background for studying signaling pathways, including Wnt, and are amenable to imaging, biochemical, and high-throughput applications.
DVL2 is a cytoplasmic phosphoprotein that acts as a central scaffold in Wnt signaling. Upon Wnt activation, receptors such as Frizzled and LRP5/6 bind DVL2, which polymerizes and recruits the AXIN1/GSK3??/APC destruction complex, inhibiting ??-catenin degradation. Stabilized ??-catenin enters the nucleus to activate TCF/LEF-mediated transcription of targets like MYC, CCND1, and AXIN2. In non-canonical pathways, DVL2 signals through ROR2/RYK to JNK and Rho GTPases (RhoA, RAC1), regulating cytoskeletal dynamics and cell polarity. DVL2 interacts with many partners, including CK1??, DAAM1, Vangl2, DACT1, and PRICKLE1, highlighting its role in pathway crosstalk.
In HEK293T cells, DVL2 disruption attenuates both canonical and non-canonical Wnt signaling, creating a clean background for mechanistic studies. The cell line??s high transfection efficiency enables rescue experiments with wild-type or mutant DVL2, facilitating structure-function analyses. This model is valuable for investigating Wnt-dependent processes such as ??-catenin stabilization, target gene induction, and EMT, as well as cross-talk with pathways like Hippo signaling. The polyclonal format minimizes clonal artifacts while supporting population-level readouts.
The DVL2 knockout polyclonal cells are suited for Wnt reporter assays (TOPFlash/FOPFlash), immunoblotting for active ??-catenin and downstream targets, co-immunoprecipitation of DVL2 complexes, and phospho-signaling analysis (JNK, c-Jun). They enable cancer research, drug screening for Wnt inhibitors, and studies of cell migration and polarity. For further information, please contact Ascent Research.
