The DYNC2H1 Knockout MES-OV Polyclonal Cells product delivers a CRISPR/Cas9-edited heterogeneous population of MES-OV cells with targeted disruption of the DYNC2H1 gene. As a polyclonal knockout pool, it maintains genetic diversity while uniformly lacking functional DYNC2H1 protein, enabling loss-of-function studies free from clonal selection biases. This model provides a robust foundation for investigating DYNC2H1-dependent processes in a human ovarian cancer background, supporting reproducible and scalable experimental designs.
The MES-OV cell line is a well-characterized adherent human ovarian clear cell carcinoma line established from a patient with ovarian adenocarcinoma. As a representative model of this epithelial cancer subtype, MES-OV cells retain key genomic and phenotypic features of the primary tumor. Their adherent growth properties facilitate a wide range of cell-based assays, including high-content imaging and functional analyses, making them a versatile host for gene editing.
DYNC2H1 encodes the heavy chain of cytoplasmic dynein 2, the motor responsible for retrograde intraflagellar transport (IFT) within primary cilia. This protein functions in a complex with light chains DYNC2LI1, DYNC2LI2 and IFT components IFT140 and IFT122 to drive the turnaround of IFT trains from the ciliary tip. DYNC2H1 activity is critical for ciliary assembly and Hedgehog (Hh) signal transduction. In the Hh pathway, receptor PTCH1 and transducer SMO regulate GLI transcription factors (GLI1?C3). DYNC2H1-mediated retrograde transport, activated by RFX transcription factors and ciliogenesis signals, is essential for trafficking signal modulators and sustaining proper pathway output.
In ovarian clear cell carcinoma, the contributions of primary cilia and DYNC2H1 to oncogenic processes are not fully defined. The DYNC2H1 knockout in MES-OV cells offers a tool to dissect cilia-dependent Hh signaling and its potential roles in proliferation, migration, and chemoresistance. This model allows separation of cilium-related functions from other cytoplasmic dynein roles, facilitating focused mechanistic studies.
Applications of these polyclonal DYNC2H1 knockout MES-OV cells include immunofluorescence-based analysis of cilia markers (e.g., ARL13B, acetylated ??-tubulin), western blot confirmation of DYNC2H1 loss, and Hedgehog reporter assays with GLI-responsive luciferase. The model also supports RT-qPCR quantification of Hh targets, cilia morphology studies, and migration/invasion assays to evaluate metastatic traits. For additional details, contact Ascent Research.
