The DYNLT3 Knockout MES-OV Polyclonal Cells comprise a CRISPR/Cas9-edited polyclonal knockout cell population derived from MES-OV murine embryonic stem cells, designed to disrupt the DYNLT3 gene encoding the dynein light chain Tctex-type 3. This loss-of-function model serves as a versatile tool for investigating cytoplasmic dynein-mediated intracellular transport and associated regulatory networks.
MES-OV cells are pluripotent embryonic stem cells established from the inner cell mass of 129/SvEv mouse blastocysts. They retain the ability to self-renew and can be directed to differentiate into oocyte-like cells, providing a physiologically relevant system for studying germ cell development, meiosis, and early embryogenesis. Their robust growth and well-characterized differentiation protocols make them ideal for generating gene-edited populations with high experimental reproducibility.
DYNLT3 functions as a light chain component of the cytoplasmic dynein complex, essential for minus-end-directed microtubule transport. It facilitates cargo attachment and regulates motor processivity by forming interactions with the dynein heavy chain DYNCH1, the intermediate chain DYNC1LI1, the dynactin complex, the adaptor BICD2, and various RAB GTPases including Rab7 and Rab11. Upstream, DYNLT3 activity is modulated by CDK1-mediated phosphorylation and cargo adaptor proteins, while downstream it governs organelle positioning, mitotic spindle orientation, and vesicle trafficking along microtubules.
In the MES-OV context, disruption of DYNLT3 perturbs dynein-based transport mechanisms critical for proper organelle distribution and spindle alignment during cell division and differentiation. Given the importance of dynein in ciliogenesis and intracellular trafficking, this knockout model enables dissection of pathways linking dynein dysfunction to ciliopathies, developmental disorders, and potentially cancer metastasis. The pluripotent nature of MES-OV cells allows exploration of how loss of DYNLT3 impacts lineage commitment and germ cell specification.
Researchers can employ this polyclonal knockout population in a broad array of functional assays, including western blotting and immunofluorescence microscopy to confirm target protein depletion, live-cell imaging to visualize real-time organelle movement defects, co-immunoprecipitation to assess binding partner alterations, and cell cycle synchronization studies combined with microtubule binding assays to evaluate mitotic spindle mechanics. It is also applicable to the analysis of ciliogenesis and cargo-specific trafficking pathways. For more details, please contact Ascent Research.
