DYNLT3 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from Raji B lymphocytes, providing a heterogeneous loss-of-function model for the DYNLT3 gene. This polyclonal format avoids clonal bias and is ideal for population-based studies of dynein light chain Tctex-type function.
The Raji cell line is a suspension-adapted human Burkitt??s lymphoma line that is positive for Epstein-Barr virus (EBV) and widely used to model B-cell malignancy, EBV latency, and lymphoid signaling. Its rapid growth and well-documented oncogenic pathways, such as NF-??B and PI3K/AKT, establish a robust platform for investigating gene function in B-cell biology.
DYNLT3 encodes a light chain of cytoplasmic dynein-1, which drives retrograde transport along microtubules and organizes the mitotic spindle. The protein is regulated by CDK1 and PLK1 phosphorylation and interacts directly with dynein heavy chain DYNC1H1, light chains DYNLL1/2, and the dynactin complex. Cargo adaptors such as BICD2 and HOOK3 link DYNLT3 to specific payloads, enabling precise intracellular trafficking. Disruption of DYNLT3 therefore compromises dynein-dependent processes, including organelle positioning, chromosome segregation, and pericentriolar matrix assembly, with downstream consequences for mitotic checkpoint proteins and cargo adaptor availability.
In Raji cells, DYNLT3 knockout is highly relevant for probing the dynein motor??s role in lymphoma cell division and survival. Because EBV exploits host dynein for episomal maintenance, this model also permits examination of viral?Chost interactions that sustain latency. Loss of DYNLT3 may precipitate mitotic errors and genomic instability, offering a potent system to study mechanisms linking microtubule motor dysfunction to B-cell transformation.
These polyclonal knockout cells support diverse functional assays: Western blotting and RT-qPCR confirm target disruption, immunofluorescence reveals spindle and dynein localization defects, and live-cell tracking captures real-time trafficking and mitotic phenotypes. Cell cycle and apoptosis assays quantify proliferation and death responses, while co-immunoprecipitation maps altered dynein?Ccargo complexes. Such applications make the DYNLT3 knockout Raji model a valuable resource for dynein biology, cancer cell research, and drug discovery. For details, contact Ascent Research.
