The EBAG9 Knockout MES-OV Polyclonal Cells product is a CRISPR/Cas9-edited polyclonal knockout cell population derived from the MES-OV human ovarian clear cell carcinoma cell line. This pool of edited cells carries targeted disruption of the EBAG9 gene, providing a versatile loss-of-function model free from clonal selection artifacts. The polyclonal format retains genetic heterogeneity while ensuring robust knockout across the population, suitable for assays requiring representative cellular behavior.
MES-OV is a human ovarian clear cell carcinoma cell line, a chemoresistant subtype of epithelial ovarian cancer. It exhibits aberrant estrogen responsiveness and dysregulated apoptosis, recapitulating key aspects of ovarian cancer biology and serving as a widely used model for disease progression and therapeutic studies.
EBAG9 encodes the tumor-associated antigen RCAS1. EBAG9 transcription is activated by ER?? upon estrogen stimulation, integrating estrogen signaling. RCAS1 binds a putative lymphocyte receptor to induce apoptosis in T cells and immune effectors, promoting immune evasion. Downstream of ER??, RCAS1 induces immune cell apoptosis, linking hormone signaling to tumor immunity. Key pathway components include ER??, ER-responsive transcription, and RCAS1 receptor-mediated apoptosis.
EBAG9 knockout disrupts the RCAS1-mediated immune-inhibitory circuit, potentially restoring anti-tumor immunity. These cells enable study of T cell inhibition relief and immune cell apoptosis reduction in the tumor microenvironment. This model is relevant for dissecting estrogen-driven immune escape and testing whether targeting the ER???CEBAG9 axis reinstates immune surveillance in ovarian clear cell carcinoma.
These cells suit diverse research applications, including study of immune evasion mechanisms, screening for modulators of estrogen-dependent immune checkpoints, and dissection of estrogen signaling in apoptosis. Typical assays include western blotting for EBAG9 protein, RT-qPCR for EBAG9 mRNA, flow cytometry for RCAS1 surface expression, and immune co-culture to measure T cell apoptosis or cytotoxicity. For technical inquiries, please contact Ascent Research.
