EDIL3 Knockout K-562 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human chronic myeloid leukemia suspension line K-562. The product features disruption of the EDIL3 gene via CRISPR/Cas9-mediated gene editing, yielding a heterogeneous pool of cells with loss-of-function mutations. This polyclonal format enables functional studies without clonal isolation, providing a practical model for population-level analyses.
K-562 cells, established from a 53-year-old female with CML in blast crisis, harbor the BCR-ABL fusion oncogene and exhibit multipotent hematopoietic progenitor properties. Widely used to investigate leukemia biology and signal transduction, this suspension line offers a relevant background for examining EDIL3 function in hematopoietic malignancies and integrin-mediated adhesion pathways.
EDIL3 encodes a secreted matricellular protein that binds integrin receptors ??v??3 and ??v??5, activating focal adhesion kinase (FAK/PTK2) and downstream SRC?CPI3K?CAKT and HRAS?CRAF1?CMAP2K1?CMAPK3 cascades to promote cell adhesion, migration, and angiogenesis. EDIL3 transcription is induced by HIF1A, TNF, IL1B, TGFB, and VEGF. The protein interacts with extracellular matrix components such as fibronectin and vitronectin, and its engagement of integrins triggers phosphorylation of key signaling nodes including AKT1 and ERK.
In the K-562 background, EDIL3 knockout disrupts autocrine/paracrine integrin signaling, enabling dissection of EDIL3-dependent adhesion dynamics independent of endothelial-specific outputs. The multipotent hematopoietic character allows exploration of EDIL3??s role in progenitor adhesion and the leukemic microenvironment, while conditioned media from these cells can be used to test paracrine effects on stromal or vascular cells.
Researchers can apply EDIL3 Knockout K-562 Polyclonal Cells for functional studies of EDIL3 in tumor angiogenesis via tube formation assays with conditioned media, or for investigation of integrin signaling through phospho-FAK/AKT analysis and cell adhesion assays. The model is suited for drug screening, RNA-seq profiling, and analysis of EDIL3 secretion by ELISA. For additional information, contact Ascent Research.
