The EEF1A2 Knockout K-562 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population derived from human chronic myelogenous leukemia K-562 cells with targeted disruption of the EEF1A2 gene. This loss-of-function model enables investigation of the tissue-specific translation elongation factor eEF1A2 in leukemia-relevant processes without clonal selection bias.
The K-562 line, established from a 53-year-old female with CML in blast crisis, contains the Philadelphia chromosome and grows as a highly proliferative suspension culture. It serves as a classic model for studying erythroid and myeloid differentiation, oncogenic signal transduction, and drug sensitivity, offering a physiologically relevant hematopoietic context for EEF1A2 knockout studies.
EEF1A2 encodes eEF1A2, which catalyses GTP-dependent aminoacyl-tRNA delivery to the ribosome. This isoform also contributes to actin cytoskeleton organization, apoptosis inhibition, and oncogenic signaling. Upstream regulators include MYC, STAT3, AKT, mTOR, and ER stress sensors; downstream targets encompass ribosomal translation machinery, ??-actin, and apoptotic regulators like BAX and Bcl-2. Interaction partners include actin filaments, ZPR1, aminoacyl-tRNA, GTP, humanin, and HIV-1 Tat. Within signaling networks, EEF1A2 intersects with the PI3K/AKT/mTOR axis, involving mTOR, AKT, PI3K, S6K1, 4E-BP1, BAX, and Bcl-2. EEF1A2 disruption impairs translation elongation and disrupts cytoskeletal and anti-apoptotic functions, sensitizing cells to apoptotic stimuli.
In K-562 cells, EEF1A2 knockout permits dissection of oncogene addiction and apoptosis regulation in CML. Loss of eEF1A2-mediated translation and survival signaling may alter proliferation and differentiation, providing a platform to study cross-talk between protein synthesis control and cytoskeletal dynamics. This model is relevant for validating EEF1A2 as a target in leukemias and other cancers with dysregulated expression.
These polyclonal knockout cells support a variety of experimental approaches, including functional analysis of EEF1A2 in cancer biology, oncogene addiction studies, drug target validation, and mechanistic investigation of apoptosis and translation regulation. Compatible assays include western blotting, RT-qPCR, cell viability (MTT/CCK-8), Annexin V/PI apoptosis detection, colony formation, puromycin incorporation translation assays, co-immunoprecipitation, immunofluorescence for actin cytoskeleton, and transcriptomic profiling by RNA-seq. For expert technical support or custom project inquiries, please contact Ascent Research.
