EEF1A2 Knockout NCI-H1299 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population generated by targeted disruption of the EEF1A2 gene in the NCI-H1299 non-small cell lung cancer cell line. This heterogeneous pool of knockout cells avoids clonal selection artifacts, providing a robust loss-of-function model for studying EEF1A2 biology. The ready-to-use polyclonal population is suitable for a broad range of functional assays in cancer biology and translational research.
The NCI-H1299 host line is derived from a lymph node metastasis of lung adenocarcinoma and serves as a widely used model for non-small cell lung cancer (NSCLC). These p53-deficient epithelial cells exhibit high proliferation and metastatic potential, making them valuable for investigating mechanisms of tumor progression, metastasis, and drug resistance in aggressive lung adenocarcinoma.
EEF1A2 encodes the eukaryotic translation elongation factor 1 alpha 2 (eEF1A2), which delivers aminoacyl-tRNA to the ribosome during mRNA translation. Beyond its canonical function, eEF1A2 participates in non-canonical roles including actin cytoskeleton organization and regulation of apoptosis. Its expression is activated by c-MYC and mTORC1 signaling, and the protein interacts with ribosomes, actin, PI3K, and SH3 domain-containing proteins. Disruption of EEF1A2 impairs global protein synthesis, alters actin dynamics, and attenuates oncogenic PI3K/AKT signaling, thereby coupling growth factor inputs to translational control and cell survival pathways involving eEF2 and AKT.
In the context of NCI-H1299 lung adenocarcinoma cells, loss of EEF1A2 is expected to reduce cell proliferation and survival, as these p53-deficient cells may depend on EEF1A2-mediated translation to sustain oncogenic programs. This polyclonal knockout model thus enables the dissection of EEF1A2-dependent molecular mechanisms in an aggressive NSCLC background, facilitating studies on how aberrant translation contributes to metastasis and drug resistance.
These polyclonal knockout cells are suitable for a variety of functional assays, including western blotting for pathway component analysis, puromycin incorporation translation assays to measure protein synthesis rates, and immunofluorescence to visualize actin cytoskeleton organization. Proliferation (MTT), apoptosis (caspase-3 activation), and transwell migration assays further clarify the impact of EEF1A2 loss on cell behavior, while drug sensitivity testing supports the identification of compounds that target EEF1A2-dependent vulnerabilities. These applications position the product as a versatile tool for studying translation regulation in lung adenocarcinoma, dissecting PI3K/AKT signaling, and screening agents directed against elongation factors. For additional information, please contact Ascent Research.
