The EEF1A2 Knockout NCI-H1975 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population that provides a loss-of-function model for the translation elongation factor EEF1A2 in a human lung adenocarcinoma background. This product enables pooled functional studies by capturing the genetic heterogeneity inherent to a polyclonal knockout format, avoiding the selective pressure of clonal isolation and offering a robust starting point for investigating the roles of EEF1A2 in cancer cell biology.
The NCI-H1975 host cell line was derived from a 62-year-old female with non-small cell lung adenocarcinoma and harbors both EGFR L858R and T790M mutations. The L858R mutation confers constitutive kinase activation, while the T790M substitution confers resistance to first-generation EGFR tyrosine kinase inhibitors. As a well-characterized epithelial cancer model, NCI-H1975 is widely used to study oncogenic signaling, drug resistance, and translational regulation in EGFR-mutant lung adenocarcinoma.
EEF1A2 encodes a GTP-dependent translation elongation factor that delivers aminoacyl-tRNAs to the ribosomal A site during protein synthesis. Its expression is transcriptionally activated by c-Myc and the E2F1 transcription factor, and it functions downstream of mTOR signaling through the mTOR?CS6K?C4E-BP1 axis. EEF1A2 interacts with components of the eEF1B complex, aminoacyl-tRNA synthetases, and the actin cytoskeleton to facilitate peptide chain elongation and modulate cytoskeletal dynamics. Downstream, EEF1A2 promotes the synthesis of pro-survival proteins such as Bcl-xL, and its activity intersects with ER stress responses and apoptosis regulation.
In the NCI-H1975 context, dysregulated mTOR signaling and high translational output are hallmarks of the malignant phenotype. EEF1A2 knockout in this EGFR-mutant background may disrupt the synthesis of critical survival factors, thereby sensitizing cells to apoptosis and providing a platform to probe the link between elongation-driven translation and drug resistance. This polyclonal knockout population offers a relevant system to dissect how EEF1A2 contributes to oncogenic translation and survival signaling in lung adenocarcinoma.
Representative research applications include Western blotting and RT-qPCR for EEF1A2 expression analysis, puromycin incorporation assays to measure de novo protein synthesis, cell proliferation and annexin V apoptosis assays, polysome profiling for ribosome occupancy, RNA-seq for transcriptome-wide effects, and drug sensitivity testing with translation inhibitors. These assays support functional annotation of EEF1A2, target validation, and exploration of resistance mechanisms. For additional details or to request a quotation, please contact Ascent Research.
