The EEF1E1 Knockout HGC-27 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population of the human gastric adenocarcinoma line HGC-27, designed to disrupt the EEF1E1 (AIMP3) gene. This loss-of-function model enables researchers to study the tumor-suppressive functions of EEF1E1. The polyclonal format provides a heterogeneous knockout pool, suitable for functional assays without clonal selection biases.
HGC-27 cells were derived from a lymph node metastasis of a poorly differentiated gastric adenocarcinoma and carry a TP53 mutation. This aggressive, metastatic model is widely used in gastric cancer research, particularly for investigating drug sensitivity and tumor progression mechanisms. The TP53-mutant background allows dissection of EEF1E1 functions in a setting of compromised p53 tumor suppressor activity.
EEF1E1 is a component of the multi-tRNA synthetase complex, yet its tumor suppressor role is exerted through the DNA damage response. Genotoxic stress triggers ATM/ATR-mediated phosphorylation of EEF1E1, prompting nuclear translocation where it binds and stabilizes p53 by blocking MDM2-dependent degradation. Stabilized p53 transcriptionally activates p21 (CDKN1A) for cell cycle arrest, and Bax and PUMA (BBC3) for apoptosis. EEF1E1 also interacts with other synthetase complex members (EPRS, AIMP1, AIMP2), but the ATM-ATR-EEF1E1-p53 axis is critical for its tumor suppression.
In HGC-27 cells with mutant TP53, loss of EEF1E1 can reveal p53-dependent and -independent pathways. This model is instrumental for studying DNA damage responses, apoptosis, and chemosensitivity in gastric cancer. It enables identification of synthetic lethal interactions and assessment of genotoxic agent efficacy. The polyclonal knockout population offers a relevant platform for gastric cancer research focused on DNA repair and cell death mechanisms.
Applications include Western blotting for p53, p21, ??H2AX; immunofluorescence for p53 localization; flow cytometry for apoptosis and cell cycle profiling; colony formation and MTT viability assays following etoposide or ??-irradiation. Co-immunoprecipitation can probe EEF1E1-p53 interactions, and RNA-seq can reveal global transcriptional changes. This product supports synthetic lethality screens and drug repurposing studies. For inquiries or custom projects, contact Ascent Research.
