EEIG2 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the early estrogen-induced gene 2 (EEIG2) in HEK293T human cells. This loss-of-function model enables investigation of EEIG2, a transcriptional coregulator involved in estrogen receptor signaling and proliferation. The polyclonal nature ensures population-level studies without clonal artifacts, suitable for consistent knockout across passages.
The host cell line, HEK293T, is an adherent human embryonic kidney epithelial line expressing SV40 large T antigen. Derived from HEK293 cells transformed with adenovirus 5 DNA, it supports high transfection efficiency and episomal replication of SV40 origin-containing plasmids. Widely used for protein production, viral packaging, and functional genomics, HEK293T offers well-characterized biology and ease of manipulation.
EEIG2, an estrogen-responsive gene, functions as a transcriptional coactivator that enhances estrogen receptor alpha (ER??) activity. Upon estrogen stimulation, EEIG2 is rapidly induced and recruited to ER?? target genes, where it interacts with coregulators such as SRC family coactivators, CBP/p300, and the Mediator complex to form an active transcriptional complex. Upstream regulators include estrogen, ER??, MAPK/ERK, and PI3K/AKT signaling. This complex drives expression of downstream proliferation-associated genes including cyclin D1 and c-Myc, as well as the anti-apoptotic factor Bcl-2, thereby promoting cell cycle progression through the cyclin D1-CDK4/6 axis.
In the HEK293T background, which lacks robust endogenous ER?? expression, this EEIG2 knockout serves as a versatile platform for dissecting ER??-dependent and -independent functions. Through ectopic pathway reconstitution, researchers can examine EEIG2 contributions to transcriptional regulation and proliferation control. This model is valuable for studying oncogenic processes, particularly in estrogen-driven breast cancer, and for screening modulators of EEIG2-ER?? interactions.
These cells can be employed in estrogen signaling pathway dissection, breast cancer oncogenesis studies, transcriptional regulation analyses, and cell cycle investigations. Representative techniques suitable for these polyclonal knockout cells include Western blotting and RT-qPCR to confirm EEIG2 disruption and assess target gene expression, luciferase reporter assays for monitoring ER?? transcriptional activity, immunofluorescence for subcellular localization, co-immunoprecipitation for protein interaction studies, cell proliferation assays (e.g., MTT, BrdU), and flow cytometry for cell cycle distribution analysis. For additional information or custom orders, please contact Ascent Research.
