The EFCAB14 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population engineered for loss-of-function studies of the EF-hand calcium-binding protein EFCAB14. This product provides a heterogeneous pool of gene-disrupted HEK293T cells suitable for functional genomics, calcium signaling research, and pooled CRISPR screens. The cells are delivered as a ready-to-use population that has undergone quality control to confirm target gene disruption while retaining the robust growth and transfection characteristics of the parental line.
HEK293T cells are a transformed human embryonic kidney epithelial cell line widely employed for recombinant expression, viral vector production, and CRISPR-based functional studies. They stably express the SV40 large T-antigen, enabling episomal replication of plasmids bearing the SV40 origin and ensuring high transfection efficiency. This adherent cell line exhibits rapid proliferation, is amenable to a variety of chemical and electroporation-based transfection methods, and maintains key signaling modules, including calcium-responsive pathways, making it a convenient chassis for investigating gene function.
EFCAB14 belongs to the EF-hand calcium-binding protein family and is predicted to act as a calcium sensor or buffer within the cell. Its mechanistic role may involve calcium-dependent interactions with calmodulin, potentially modulating calmodulin-regulated effectors such as CaMKII and calcineurin. Upstream regulation could occur through calcium-sensitive transcription factors like CREB and NFAT, which respond to intracellular calcium elevations. Although its direct downstream targets are unknown, EFCAB14 is postulated to participate in calcium ion homeostasis and the fine-tuning of calcium signal transduction dynamics.
Disruption of EFCAB14 in HEK293T cells enables investigation of its function in a well-characterized epithelial environment that endogenously expresses core calcium signaling components. The polyclonal knockout format eliminates clonal variability and simplifies pooled assay workflows, making it ideal for high-throughput applications such as CRISPR suppressor screens or interactome profiling. By comparing this polyclonal population with wild-type HEK293T cells, researchers can delineate EFCAB14-dependent changes in calcium flux, gene expression, and protein network interactions under physiologically relevant conditions.
This knockout cell pool supports diverse applications, including functional characterization of EF-hand proteins, calcium signaling analysis, and systematic protein?Cprotein interaction studies. Representative experimental approaches comprise RT-qPCR and immunoblotting for knockout validation, Fura-2-based calcium imaging, co-immunoprecipitation with calmodulin, and transcriptomic profiling via RNA-seq. Additional phenotypic assays may assess proliferation, apoptosis, or responses to calcium agonists. The cells provide a versatile platform for dissecting EFCAB14 biology. For more information, please contact Ascent Research.
