EFCAB14 Knockout MES-OV Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population with targeted disruption of the EFCAB14 gene in the MES-OV human ovarian clear cell carcinoma cell line. This polyclonal format provides a heterogeneous cell population lacking EFCAB14 expression, enabling studies of EFCAB14 function without clonal bias. The CRISPR/Cas9 system was used to introduce loss-of-function mutations at the endogenous locus, generating a mixed population of knockout cells suitable for population-level analyses.
The MES-OV cell line, derived from a patient with ovarian clear cell carcinoma, exhibits adherent epithelial morphology and retains characteristics of the original tumor. It serves as a widely used model for investigating the molecular pathogenesis of ovarian clear cell carcinoma, including signaling alterations that drive tumor progression and therapy resistance. The clinically relevant background of MES-OV makes it an ideal host for functional genomics studies in ovarian cancer.
EFCAB14 encodes an EF-hand calcium-binding protein that functions as a calcium sensor. Upon binding intracellular calcium mobilized by upstream G?protein coupled receptors and growth factor receptors, EFCAB14 interacts with calmodulin, other EF-hand proteins, and protein kinases to activate downstream effectors, including calmodulin-dependent kinases, calcineurin, and transcriptional regulators. The calcium signaling pathway involves phospholipase C, inositol triphosphate receptor, and protein kinase C, which together coordinate cellular responses. Thus, EFCAB14 links extracellular signals to intracellular calcium-dependent transcriptional and post-translational regulation.
In MES-OV cells, knockout of EFCAB14 enables dissection of calcium-dependent pathways in ovarian clear cell carcinoma. This subtype exhibits distinct signaling dependencies, and EFCAB14 loss may perturb calcium-mediated regulation of proliferation, migration, and survival. By evaluating changes in downstream effectors such as calmodulin-dependent kinases and calcineurin, researchers can elucidate mechanisms underlying ovarian cancer pathogenesis and identify potential therapeutic targets.
These polyclonal knockout cells are intended for functional characterization of EFCAB14, calcium signaling studies, drug target validation, and tumorigenesis analyses. Common experimental techniques include western blotting, RT-qPCR, intracellular calcium imaging with Fluo?4, cell proliferation and migration assays, and RNA?seq. This model supports detailed molecular and phenotypic investigations. For more information or to inquire about custom configurations, contact Ascent Research.
