The EFEMP1 Knockout HGC-27 Polyclonal Cells product is a CRISPR/Cas9-edited polyclonal cell population derived from the human gastric carcinoma HGC-27 cell line, featuring targeted disruption of the EFEMP1 gene that encodes the extracellular matrix glycoprotein fibulin-3. The polyclonal knockout cells are supplied as a heterogeneous pool of edited cells, generated via CRISPR/Cas9-mediated gene disruption, enabling loss-of-function studies without the need for single-cell cloning. This format is suitable for functional investigations where population-level effects of gene disruption are desired.
HGC-27 is a human gastric carcinoma cell line originally established from a lymph node metastasis of a male patient, widely employed as a model for gastric cancer invasion and metastatic dissemination. The cells exhibit mesenchymal characteristics and are used to assess tumor cell motility, ECM degradation, and metastatic colonization. The parental HGC-27 line provides a relevant background for investigating the role of EFEMP1 in gastric cancer pathology.
EFEMP1 encodes fibulin-3, a secreted ECM glycoprotein involved in cell adhesion and migration through interactions with fibronectin, laminin, and integrin ??V??3. Fibulin-3 modulates integrin-mediated focal adhesion dynamics and downstream FAK phosphorylation and cytoskeletal reorganization. EFEMP1 also influences TGF-?? and Wnt pathways by affecting SMAD2/3 transcriptional activity and ??-catenin stability. Consequently, fibulin-3 regulates ECM remodeling and MMP expression, impacting tissue architecture and invasion.
Disruption of EFEMP1 in HGC-27 cells is anticipated to perturb fibulin-3-dependent cell?CECM adhesion and intracellular signaling, providing a powerful model to dissect the molecular mechanisms driving gastric cancer cell invasion. Loss of fibulin-3 may reduce integrin-mediated attachment and alter FAK and SMAD signaling, potentially leading to changes in cell motility, MMP secretion, and metastatic behavior. By studying the EFEMP1 knockout HGC-27 polyclonal cells, researchers can evaluate the functional consequences of fibulin-3 deficiency within the context of a well-characterized gastric carcinoma metastatic background, thereby illuminating its role in tumor progression and ECM remodeling.
These polyclonal knockout cells are ideally suited for a range of functional assays aimed at understanding gastric cancer biology. Typical applications include Transwell migration and Matrigel invasion assays to quantify invasive potential, Western blotting and RT-qPCR to analyze expression of key signaling molecules such as phospho-FAK, SMAD2/3, ??-catenin, and various MMPs, and immunofluorescence to assess focal adhesion architecture and fibronectin organization. Furthermore, the model can be employed in drug sensitivity screens to identify compounds that differentially affect EFEMP1-deficient gastric cancer cells. The EFEMP1 knockout HGC-27 cells thus serve as a versatile tool for dissecting ECM-driven signaling and metastatic mechanisms. For detailed technical specifications and ordering information, please contact Ascent Research.
