EGFLAM Knockout HAP1 Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Bone Marrow
Disease:
Chronic myeloid leukemia

Datasheet

The EGFLAM Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population in the HAP1 near-haploid cell line, targeting the EGFLAM gene that encodes pikachurin. Pikachurin is an extracellular matrix protein that binds dystroglycan and plays a critical role in retinal photoreceptor ribbon synapse formation, with direct relevance to muscular dystrophy-dystroglycanopathy. Applications include western blotting for dystroglycan glycosylation, co-immunoprecipitation of pikachurin?Cdystroglycan complexes, and cell adhesion assays. This polyclonal knockout pool is ideal for robust gene disruption studies in dystroglycan signaling and synaptic biology research.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

HAP1

Sex of Donor

Male

Age

40 years

Derived From Site

Bone marrow

Gene Name

EGFLAM

Gene Identifier

NCBI Gene ID 133584

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

IMDM

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The EGFLAM Knockout HAP1 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human near-haploid HAP1 cell line, engineered to disrupt the EGFLAM gene.

This loss-of-function model provides a versatile system for investigating EGFLAM-dependent processes without the constraints of a single clonal isolate, capturing a spectrum of genetic edits across the population.

The polyclonal nature facilitates assessment of gene disruption effects while mitigating clonal artifacts that can arise in monoclonal knockout lines, offering a robust approach for functional genomics studies.