The EHBP1 Knockout SK-HEP-1 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal population with targeted disruption of the EHBP1 gene in the SK-HEP-1 human hepatic adenocarcinoma cell line. This polyclonal format provides a heterogeneous gene-disrupted pool, serving as a robust loss-of-function model for studying EHBP1-dependent pathways without clonal selection biases. The knockout eliminates functional EHBP1 protein, enabling precise investigation of its roles in endocytic trafficking and actin cytoskeleton dynamics.
SK-HEP-1 is a well-established human hepatic adenocarcinoma cell line exhibiting endothelial-like characteristics, including endothelial marker expression and capillary-like tube formation in vitro. Isolated from ascites of a liver adenocarcinoma patient, it represents a unique hybrid model for hepatocellular carcinoma and endothelial biology studies. This background is particularly valuable for investigating processes at the tumor-endothelial interface, such as transendothelial migration and angiogenesis.
EHBP1 is a scaffold integrating endocytic recycling with actin remodeling. It binds EHD1, EHD2, and OCRL, linking cargo sorting to phosphoinositide metabolism. Activated downstream of Rac1, Cdc42, and receptor tyrosine kinases, EHBP1 recruits N-WASP to promote actin polymerization at endosomes. This facilitates recycling of internalized cargo and maintains cell polarity. Knockout disrupts these interactions, impairing endosomal trafficking and actin dynamics, leading to enhanced cell migration defects and invasive capacity.
In the SK-HEP-1 context, EHBP1 knockout provides a powerful tool to dissect the contribution of endosomal recycling to hepatocellular carcinoma progression. The endothelial-like properties of this cell line allow researchers to explore how EHBP1-dependent trafficking influences transendothelial migration, a critical step in metastasis. Furthermore, the polyclonal nature of the knockout population more closely mirrors the genetic heterogeneity observed in tumors, making it especially suited for studying cancer cell invasion and metastasis mechanisms.
These cells are ideal for transwell migration/invasion, transferrin recycling, co-IP/Western for EHD1, immunofluorescence for actin/endosomes, live-cell imaging, phospho-signaling for Rac1/Cdc42, and RNA-seq. They enable research in metastasis, endosomal trafficking, and actin dynamics. For further details, contact Ascent Research.
