The EHD1 Knockout HEK293T Polyclonal Cells constitute a CRISPR/Cas9-mediated gene-disrupted polyclonal knockout cell population targeting the EHD1 locus in the HEK293T human embryonic kidney epithelial cell line. This product provides a loss-of-function model generated through CRISPR/Cas9 genome editing, yielding a heterogeneous pool of edited cells suitable for studying the cellular consequences of impaired endocytic recycling. As a polyclonal population, it avoids the clonal variation often associated with single-cell-derived knockouts, offering a broadly representative mutant cell pool for functional analyses.
HEK293T cells, a derivative of the original HEK293 line, are immortalized adherent epithelial cells transformed with sheared adenovirus 5 DNA. They stably express the SV40 large T antigen, which drives episomal amplification of plasmids bearing the SV40 origin, making them a premier host for transient protein expression and efficient lentivirus production. The cell line??s robust growth, high transfection efficiency, and well-characterized signaling milieu underpin its widespread use in cell biology and drug discovery.
EHD1 is a dynamin-like ATPase that orchestrates endocytic recycling by promoting fission of tubular carriers from early endosomes, facilitating the return of internalized receptors and lipids to the plasma membrane. It operates within a network that includes upstream regulators such as EGF and insulin, interacting partners like Rab11, MICAL-L1, Syndapin, and the EHD paralogs EHD2, EHD3, and EHD4, and downstream targets including the transferrin receptor, beta1 integrin, and various GPCRs. EHD1-dependent recycling is essential for maintaining plasma membrane receptor composition and supporting processes such as cell migration and signal transduction.
In HEK293T cells, disruption of EHD1 function impairs the efficient re?presentation of the transferrin receptor and beta1 integrin at the cell surface, leading to defects in iron uptake and integrin-dependent adhesion and migration. Given the HEK293T cell??s role as a workhorse for recombinant protein production and viral packaging, the knockout model also provides a platform to examine how membrane trafficking perturbations influence protein expression, secretion, and lentivirus vector assembly.
This polyclonal knockout cell product enables a wide array of research applications, including quantitative transferrin uptake and recycling assays, receptor internalization kinetics, cell migration analyses, and co?immunoprecipitation studies to map EHD1 interactomes. It is well suited for probing clathrin-independent endocytosis, GPCR trafficking, and the impact of recycling defects on cancer cell motility or drug delivery strategies. For additional details and ordering information, please contact Ascent Research.
