The EHD1 Knockout SK-HEP-1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population generated from SK-HEP-1 human hepatic adenocarcinoma cells. This polyclonal pool contains a heterogeneous spectrum of EHD1 gene disruptions, providing a loss-of-function model for studying endocytic recycling without clonal selection bias. The product is designed for researchers investigating receptor trafficking, membrane dynamics, and downstream signaling pathways that depend on EHD1 activity.
SK-HEP-1 is an ascites-derived cell line retaining features of liver sinusoidal endothelial cells, including roles in filtration, endocytosis, and immune cell interaction. Its endothelial-like properties and malignant origin make it a robust model for liver cancer biology, tumor microenvironment studies, and investigations of sinusoidal cell function. The expression of scavenger receptors and adhesion molecules supports research on metastatic dissemination and drug uptake mechanisms.
EHD1 encodes an ATPase that promotes vesicle fission from the endocytic recycling compartment, facilitating the return of internalized receptors to the plasma membrane. This process is regulated by membrane curvature, Arf6, Rab11, and PI3K signaling, and EHD1 directly interacts with Syndapin, Rab11-FIP2, and EHD2/EHD3/EHD4. Key downstream targets include EGFR, integrin beta1, transferrin receptor, and GLUT4. Disruption of EHD1 impairs recycling of these receptors, thereby modulating proliferative, migratory, and metabolic signaling pathways.
In SK-HEP-1 cells, loss of EHD1 allows dissection of how endocytic recycling governs liver sinusoidal endothelial functions such as receptor-mediated endocytosis, transcytosis, and immune cell engagement. The model is pertinent to cancer metastasis research, as EHD1-dependent integrin trafficking influences cell adhesion and invasion. Additionally, impaired GLUT4 recycling links to metabolic disorders, and altered EGFR trafficking affects oncogenic signaling, making this knockout population valuable for cancer biology and drug discovery.
This polyclonal knockout product is suitable for transferrin recycling and EGFR internalization assays, cell migration/invasion studies, western blotting, immunofluorescence, co-immunoprecipitation, flow cytometry, and RT-qPCR. Researchers in viral entry, drug delivery, and receptor trafficking will find it a versatile tool for mechanistic and screening studies. For further information and technical support, please contact Ascent Research.
