The EHD3 Knockout MES-OV Polyclonal Cells are a CRISPR/Cas9-mediated gene disruption model, offering a polyclonal knockout cell population derived from the MES-OV human ovarian carcinoma cell line. This polyclonal format avoids biases associated with single-cell cloning, providing a diverse pool of cells with EHD3 loss-of-function for robust and reproducible studies of endocytic trafficking pathways.
The MES-OV cell line is an established model of human ovarian carcinoma, retaining key oncogenic signaling networks and epithelial characteristics relevant to ovarian cancer biology. Its use as the host for EHD3 knockout enables investigation of endosomal recycling mechanisms in the context of ovarian cancer pathogenesis, including altered receptor trafficking and cell migration typically observed in malignant progression.
EHD3 encodes an ATPase that functions as a key regulator of endocytic recycling, controlling the trafficking of multiple receptors including EGFR, transferrin receptor, integrin ??5??1, and the cardiac sodium channel NaV1.5. EHD3 interacts with endosomal machinery components such as Rab11a, Rab11-FIP2, and Arf6, as well as syndapin, amphiphysin, and ankyrin-G. Upstream regulators include EGF, DNMT1, and HIF1A, while downstream consequences of EHD3 activity involve modulation of EGFR surface expression, transferrin receptor recycling, and integrin-dependent cell migration and proliferation. Disruption of EHD3 impairs these recycling routes, leading to altered signaling outputs and cellular responses.
In the MES-OV ovarian cancer background, EHD3 knockout is expected to perturb EGFR and integrin trafficking, thereby affecting key oncogenic processes such as proliferation, migration, and invasion. This model enables the dissection of endosomal recycling contributions to ovarian cancer cell behavior and may help identify therapeutic vulnerabilities linked to receptor trafficking defects. It also serves as a platform to study the intersection of endocytosis and oncogenic signaling in a disease-relevant cellular environment.
Typical applications include endocytosis and receptor trafficking studies, functional analyses of EGFR and integrin dynamics, and drug discovery efforts targeting trafficking-related pathways. Experimental readouts can utilize transferrin uptake assays, immunofluorescence and flow cytometry for surface receptor quantification, western blotting for protein expression, and functional assays such as transwell migration and proliferation measurements. This polyclonal knockout population is suitable for both mechanistic investigations and high-throughput screening campaigns. For additional information, please contact Ascent Research.
