The EHD4 Knockout HGC-27 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population with disrupted EHD4 gene function, derived from the HGC-27 gastric carcinoma line. This pool of knockout cells enables loss-of-function analysis of EHD4, an endocytic recycling ATPase, while preserving the genetic heterogeneity essential for modeling tumor cell populations. Researchers can use this system to dissect endosomal trafficking pathways without clonal selection bias, facilitating robust studies of cell biological processes central to cancer progression.
HGC-27 is a poorly differentiated gastric adenocarcinoma cell line established from a lymph node metastasis, representing an aggressive model of metastatic disease. These epithelial cells are widely used to investigate mechanisms of gastric cancer invasion and to evaluate therapeutic strategies. Their metastatic origin and invasive properties make them an ideal host for interrogating genes that regulate cell adhesion, migration, and membrane receptor dynamics during cancer spread.
EHD4 is a dynamin-related ATPase that drives endocytic recycling by promoting tubulation and fission of recycling endosomes. EHD4 operates downstream of ARF6 and receptor tyrosine kinases, interacting with Syndapin, Rab11-FIP2, and actin to control the return of internalized receptors such as integrins, transferrin receptor, and EGFR to the plasma membrane. Through coordinated action with Rab11 and Rab35, EHD4 modulates endosomal membrane architecture, linking membrane trafficking to cytoskeletal reorganization required for cell motility.
In gastric cancer, dysregulated EHD4-mediated recycling contributes to altered receptor presentation and metastatic behavior. Disrupting EHD4 in HGC-27 cells offers a relevant model to examine how loss of endocytic recycling impairs integrin surface expression, EGFR trafficking, and actin dynamics??key drivers of invasion. This knockout population enables exploration of how membrane trafficking hubs influence oncogenic signaling and chemosensitivity, providing a platform to study therapeutic vulnerabilities in metastatic gastric adenocarcinoma.
The EHD4 Knockout HGC-27 Polyclonal Cells support diverse applications including Western blotting, RT-qPCR, immunofluorescence, and functional migration and invasion assays. Transferrin recycling assays, flow cytometry, and co-immunoprecipitation enable detailed analysis of receptor trafficking and protein interactions. This tool is valuable for dissecting the role of endosomal recycling in cancer metastasis and for evaluating drug candidates targeting these pathways. For additional details, please contact Ascent Research.
