The EIF2A Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human ovarian carcinoma cell line A2780. This product features targeted disruption of the EIF2A gene, encoding the alpha subunit of eukaryotic translation initiation factor 2 (eIF2). The polyclonal knockout pool provides a robust loss-of-function model for investigating the integrated stress response (ISR) and translational control in a cancer-relevant background.
The A2780 cell line is a well-characterized model of human ovarian carcinoma, originally established from an untreated patient. These adherent epithelial cells are widely used in ovarian cancer research, including studies on tumorigenesis, metastasis, and drug resistance. A2780 offers a pertinent context for examining how dysregulation of translation contributes to malignant phenotypes and therapeutic responses.
EIF2A is an essential component of the eIF2 complex, which directs initiator Met-tRNAi to the 40S ribosomal subunit during translation initiation. Under stress conditions, kinases including PERK (EIF2AK3), PKR (EIF2AK2), GCN2 (EIF2AK4), and HRI (EIF2AK1) phosphorylate eIF2??, inhibiting the guanine nucleotide exchange factor eIF2B. This reduces global protein synthesis while selectively upregulating translation of ATF4, a transcription factor that induces stress-responsive genes such as CHOP and GADD34. Disruption of EIF2A abrogates functional eIF2 complex formation, thereby attenuating ISR signaling and altering cellular adaptation to stress.
In ovarian carcinoma, ISR activation contributes to tumor cell survival under adverse conditions such as hypoxia, nutrient deprivation, and chemotherapy. EIF2A knockout in A2780 cells may sensitize them to ER stress-inducing agents like tunicamycin or shift the balance between pro-survival and pro-apoptotic outputs mediated by ATF4 and CHOP. This model thus facilitates dissection of the molecular determinants governing stress adaptation and drug resistance in ovarian cancer.
These polyclonal EIF2A-knockout A2780 cells are suitable for a range of assays. Western blotting for eIF2?? phosphorylation and ATF4 protein levels enables assessment of ISR activation. Global translation rates can be measured by puromycin incorporation, and ATF4 transcriptional activity evaluated with luciferase reporters. RT-qPCR analysis of ATF4 targets (e.g., CHOP, GADD34) validates pathway disruption, while cell viability assays under ER stress (e.g., tunicamycin) define functional consequences. For additional information, please contact Ascent Research.
