EIF2AK4 Knockout jurkat Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Blood (peripheral blood)
Disease:
Acute lymphoblastic leukemia (ALL)

Datasheet

Typical research applications include probing the ISR in T cell biology through studies of amino acid deprivation signaling, stress-induced apoptosis, and metabolic adaptation. The knockout cells can be used in assays measuring phospho-eIF2?? (Ser51), ATF4, and CHOP by western blotting; ATF4 target gene expression by RT-qPCR; and apoptosis or cell cycle alterations by flow cytometry. […]

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Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

Jurkat

Cell Type

T cell line

Sex of Donor

Male

Age

14 years

Derived From Site

In situ; Peripheral blood

Gene Name

EIF2AK4

Gene Identifier

NCBI Gene ID 440275

Growth Mode

Suspension

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

Typical research applications include probing the ISR in T cell biology through studies of amino acid deprivation signaling, stress-induced apoptosis, and metabolic adaptation.

The knockout cells can be used in assays measuring phospho-eIF2?? (Ser51), ATF4, and CHOP by western blotting; ATF4 target gene expression by RT-qPCR; and apoptosis or cell cycle alterations by flow cytometry.

Additionally, they are suitable for drug sensitivity testing with GCN2 inhibitors like GCN2iB, metabolic profiling (ATP levels, MTS), and co-immunoprecipitation of pathway components.

These applications advance understanding of cancer cell stress adaptation, neurodegenerative mechanisms, and pulmonary hypertension, supporting translational research.

For further technical information, please contact Ascent Research.