The EIF4E3 Knockout HAP1 Polyclonal Cells product comprises a CRISPR/Cas9-edited polyclonal population of HAP1 cells carrying targeted disruption of the EIF4E3 gene. This polyclonal knockout pool provides a heterogeneous loss-of-function model for studying the translational repressor EIF4E3 in a near-haploid genetic background. The product enables investigation of cap-dependent translation regulation without requiring single-cell cloning, facilitating rapid functional genomic screens and pooled knockout studies.
The HAP1 cell line is a near-haploid human cell line originally derived from the KBM-7 chronic myeloid leukemia line, characterized by a haploid karyotype for most chromosomes except a disomic region of chromosome 8. This near-haploidy simplifies genetic analysis by reducing gene redundancy, making HAP1 cells an ideal host for knockout-based functional genomics and haploid genetic screens. The leukemic origin also renders them relevant for cancer biology studies, particularly in haematological malignancies.
EIF4E3 functions as a translational repressor by competing with EIF4E for binding to the mRNA 5?? cap, thereby inhibiting cap-dependent translation initiation. Its activity is regulated by the mTORC1/4E-BP1 axis, as well as by MNK1 and MNK2 kinases that phosphorylate eIF4E family members. EIF4E3 interacts with eIF4G and the 4E-BP family, and its disruption is expected to relieve translational repression of specific mRNAs, including those with structured 5?? UTRs, cell proliferation regulators, and stress response genes.
In the HAP1 near-haploid background, EIF4E3 knockout provides a genetically tractable system to dissect the gene??s contribution to translational control and its impact on leukemic cell biology. Given the host line??s origin from chronic myeloid leukemia, this model is particularly suited to explore how EIF4E3-mediated translational repression influences proliferation, survival, and stress responses in a haematological cancer context. The polyclonal nature of the knockout pool maintains heterogeneity, allowing study of gene function without clonal artifacts.
This product is well-suited for applications including functional genomics screens, elucidation of translation regulation mechanisms, cancer biology studies, and validation of drug targets linked to the mTOR or MAPK/ERK pathways. Researchers can employ assays such as polysome profiling to assess translation efficiency, cap-binding assays to study EIF4E3 interactions, and RNA-seq to identify downstream target mRNAs. Flow cytometry and proliferation assays enable analysis of cell cycle and growth effects. For further technical information or ordering details, please contact Ascent Research.
