EIF5A2 Knockout AGS Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout population of the AGS human gastric adenocarcinoma cell line, in which the EIF5A2 gene is disrupted to ablate protein expression. This heterogeneous loss-of-function model avoids clonal artifacts, providing a pooled knockout cell product for robust functional studies. The CRISPR/Cas9-mediated gene disruption eliminates EIF5A2, enabling investigation of its roles in gastric cancer biology without the need for single-cell clone characterization.
The AGS cell line originates from an epithelial tumor of a gastric adenocarcinoma patient and is a standard model for studying gastric carcinogenesis and metastasis. These cells display aggressive growth characteristics, responsiveness to growth factors, and invasive properties, making them suitable for dissecting molecular mechanisms underlying gastric cancer progression. The epithelial nature of AGS cells renders them particularly relevant for examining epithelial-to-mesenchymal transition and migration.
EIF5A2 functions as a translation elongation factor that facilitates synthesis of proteins containing polyproline stretches and other ribosome-pausing motifs. Its activity strictly requires hypusination, mediated by deoxyhypusine synthase (DHPS) and deoxyhypusine hydroxylase (DOHH). EIF5A2 is transcriptional target of MYC and operates downstream of mTORC1, linking growth signals to protein synthesis. It interacts with ribosomal proteins, RPS3A, and eEF2 to drive translation of oncogenic mRNAs such as Cyclin D1, Snail, Bcl-2, c-Myc, and MMP9. Thus, EIF5A2 promotes proliferation, EMT, apoptosis inhibition, and migration.
In AGS gastric cancer cells, EIF5A2 knockout abolishes hypusine-dependent translation elongation of MYC-driven oncogenic transcripts, thereby impairing cell growth and invasive capacity. This model enables dissection of the eIF5A hypusination pathway and its cross-talk with mTORC1 and MYC networks in gastric adenocarcinoma. The resulting attenuation of Snail and MMP9 expression reduces mesenchymal traits, highlighting EIF5A2??s role in metastasis. The polyclonal knockout is advantageous for drug screening and systems-level analyses targeting the translational machinery.
This knockout product supports a wide spectrum of experimental applications, including western blotting for EIF5A2 and downstream targets (MYC, Snail, Cyclin D1), RT-qPCR, proliferation assays (MTS, BrdU), transwell migration/invasion, colony formation, and xenograft tumor studies. Advanced approaches such as RNA-seq, polysome profiling, immunoprecipitation for interaction partners (DHPS, DOHH), and hypusine detection offer deeper mechanistic insight. Applications span gastric cancer biology, oncogene dependence studies, drug target validation, and translational control research. For further inquiries, please contact Ascent Research.
