The EIPR1 Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell pool providing a loss-of-function model for the EARP complex subunit EIPR1. This heterogeneous population carries gene disruptions that avoid clonal artifacts and preserve biological variability. The polyclonal format ensures consistent knockout representation, supporting reproducible functional assays and high-content screening, particularly suited for investigating endosomal trafficking and receptor recycling in cancer biology.
HeLa cells are an HPV-18-positive immortalized cervical adenocarcinoma line with robust endocytic activity and well-characterized signaling. Their rapid proliferation and genetic tractability make them a preferred model for membrane trafficking studies. The active EGFR and transferrin receptor pathways in HeLa provide an optimal background for assessing EARP complex function in receptor recycling and tumor biology.
EIPR1 functions as an integral subunit of the EARP complex, which also comprises VPS51, VPS52, VPS53, and VPS54. This tethering complex docks endosome-derived vesicles onto the trans-Golgi network (TGN), coordinating retrograde transport and recycling of receptors like EGFR and transferrin receptor. EIPR1 activity is regulated by EGFR signaling and epigenetic imprinting, interacting with Rab GTPases including Rab4 and Rab11 to facilitate vesicle docking. Loss of EIPR1 disassembles the EARP complex, leading to aberrant receptor sorting and impaired retrograde trafficking.
In the HeLa context, EIPR1 knockout directly perturbs endosomal compartment dynamics, causing mislocalization of recycled receptors and enhanced EGFR degradation. This defect attenuates growth factor signaling, alters cell migration and invasion, and potentially compromises tumor-suppressive functions. Given its candidate tumor suppressor role and link to Beckwith-Wiedemann syndrome, this model offers a system to explore vesicle transport in oncogenesis. The polyclonal knockout mirrors tumor heterogeneity, increasing translational relevance.
Key applications include quantitative transferrin recycling and EGFR degradation assays, immunofluorescence colocalization with TGN markers, Western blotting, migration/invasion assays, xenograft studies, and RNA-seq. This product supports drug resistance research, tumor suppressor functional assays, and genetic interaction screens mapping endosomal trafficking networks. For detailed information, contact Ascent Research.
