The ELANE Knockout KYSE-150 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population for loss-of-function studies of the ELANE gene. This heterogeneous pool of KYSE-150 cells carries targeted disruptions of ELANE, abolishing expression of the neutrophil elastase serine protease. The polyclonal format avoids clonal selection artifacts, enabling investigation of elastase-dependent matrix degradation, inflammation, and tumor progression.
The host cell line, KYSE-150, is a poorly differentiated human esophageal squamous cell carcinoma line derived from a Japanese male patient. Widely used in cancer biology, KYSE-150 exhibits invasive properties and dysregulated signaling networks, making it a clinically relevant model for studying protease-dependent mechanisms in esophageal carcinoma.
ELANE encodes neutrophil elastase, a serine protease that degrades extracellular matrix proteins such as elastin and collagen, and processes bioactive molecules like matrix metalloproteinases, IL-1??, and IL-8 to drive inflammation and tissue remodeling. Its expression is controlled by upstream regulators including G-CSF, GM-CSF, LPS, and the transcription factors C/EBP?? and PU.1, while its proteolytic activity is antagonized by endogenous inhibitors ??1-antitrypsin and serpin B1. ELANE signaling converges on key pathways such as TLR4, NF-??B, MAPK, PI3K, and AKT, and it interacts with integrins and CD11b/CD18 to regulate cell adhesion and immune cell recruitment. Disruption of ELANE abolishes neutrophil elastase activity, impairing matrix degradation and attenuating pro-inflammatory cascades, which may suppress tumor invasion and alter leukocyte trafficking.
In KYSE-150 esophageal carcinoma cells, ELANE knockout reduces matrix degradation and pro-inflammatory cytokine production, potentially limiting tumor invasion and reshaping the tumor microenvironment. This model allows dissection of cancer cell-intrinsic roles of elastase, independent of neutrophil functions, and may uncover vulnerabilities in esophageal squamous cell carcinoma progression.
This polyclonal ELANE knockout population is ideally suited for investigating tumor microenvironment crosstalk, inflammation-driven carcinogenesis, protease-dependent signaling mechanisms, and drug target validation in esophageal carcinoma research. Researchers can employ it in a variety of functional assays, including western blotting for pathway analysis, gelatin zymography to measure matrix degradation activity, transwell invasion assays to assess cell motility, cytokine ELISAs for profiling inflammatory mediators, flow cytometry for apoptosis evaluation, and RNA-seq for global differential expression studies. By enabling detailed functional dissection of ELANE in a disease-relevant cellular context, this product accelerates discovery in oncology and inflammation biology. For additional information or to discuss custom applications, please contact Ascent Research.
