ERBB2 Knockout Raji Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Bone
Disease:
Burkitt lymphoma

Datasheet

Rigorous downstream applications are enabled by this knockout model, including quantitative Western blotting for ERBB2, phospho-AKT (Ser473), and phospho-ERK1/2 (Thr202/Tyr204) to validate pathway disruption. Cell proliferation can be assessed via MTT or CCK-8 assays, while apoptosis is measured using annexin V/PI flow cytometry. Transcriptional changes in ERBB2, MYC, and Cyclin D1 can be monitored by […]

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Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

Raji

Cell Type

B cell line

Sex of Donor

Male

Age

11 years

Derived From Site

In situ; Maxilla

Gene Name

ERBB2

Gene Identifier

NCBI Gene ID 2064

Morphology

Lymphoblast-like

Growth Mode

Suspension

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

Cell proliferation can be assessed via MTT or CCK-8 assays, while apoptosis is measured using annexin V/PI flow cytometry.

Transcriptional changes in ERBB2, MYC, and Cyclin D1 can be monitored by RT-qPCR, and ERBB2 subcellular localization can be examined via immunofluorescence.

The cells are also compatible with drug sensitivity screens for HER2-targeted therapeutics and with co-culture systems designed to explore B-cell immune functions.

Overall, this product serves as a versatile tool for cancer research, signal transduction studies, drug resistance mechanism investigations, and B-cell malignancy research. For further details or custom inquiries, please contact Ascent Research.