The FGF2 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population targeting FGF2 in Raji B lymphocytes. This loss-of-function model disrupts fibroblast growth factor 2 expression, enabling study of FGF2-dependent signaling in a hematopoietic context. The polyclonal pool avoids clonal artefacts and supports population-level functional assays and screening.
Raji cells are a B lymphocyte line from a Burkitt lymphoma patient, EBV-positive, serving as a model for lymphoma and immune signaling. EBV-driven NF-??B activation provides a relevant background for studying viral oncogenesis interplay with growth factor signaling. FGF2 knockout here aids dissection of FGF2??s role in lymphoma proliferation and survival.
FGF2 is a secreted growth factor that binds FGFR1?C4 in a heparan sulfate proteoglycan-dependent manner, inducing receptor dimerization and autophosphorylation. Adaptor proteins FRS2 and GRB2 recruit SOS and activate RAS, triggering RAF?CMEK?CERK1/2 (MAPK/ERK) signaling. Additionally, FGF2 activates PI3K?CAKT and PLC?èCPKC cascades, regulating transcription of FOS, JUN, CCND1, VEGF, and BCL2. Upstream, FGF2 expression is regulated by hypoxia (HIF-1??), TGF-??, thrombin, and inflammatory cytokines like IL-1??. These pathways collectively control proliferation, survival, migration, and angiogenesis.
In Raji cells, FGF2 knockout enables study of autocrine/paracrine growth factor loops supporting lymphoma expansion. EBV-driven signaling may synergize with FGF2-mediated MAPK/ERK and PI3K?CAKT pathways. Disruption reveals FGF2 contributions to proliferation, apoptosis resistance, and tumor microenvironment angiogenesis. This model is relevant to lymphoma, cancer angiogenesis, and EBV pathogenesis, potentially uncovering therapeutic vulnerabilities in B-cell malignancies.
Researchers can employ these cells in Western blotting to assess ERK and AKT phosphorylation, RT-qPCR for downstream targets FOS and CCND1, CFSE proliferation assays, and Annexin V apoptosis profiling. Co-culture angiogenesis assays and RNA-seq provide comprehensive functional and transcriptomic insights. These polyclonal knockout cells are valuable for drug discovery, functional genomics, and dissecting FGF2-dependent signaling networks. For additional technical details or custom bulk orders, please contact Ascent Research.
