The FNBP1L Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji B lymphocyte line. This product provides a loss-of-function model for studying FNBP1L by disrupting the gene across the population, enabling assessment of its collective impact on cell behavior without clonal artifacts. The polyclonal format is well-suited for bulk functional assays where heterogeneity reflects the edited pool, and it avoids biases associated with monoclonal selection.
Raji is an EBV-transformed B lymphoblastoid cell line from a Burkitt lymphoma patient, serving as a model for B-cell malignancies and immune function. These suspension cells express surface IgM and MHC molecules, allowing studies of antigen presentation and B-cell receptor signaling. Their rapid growth and genetic tractability make Raji a standard platform for investigating lymphoma biology and testing therapeutics.
FNBP1L is a scaffold protein that links CDC42 and Rho GTPase signaling to actin polymerization. Activated downstream of receptors such as EGFR, FNBP1L recruits N-WASP and formins (FMNL2, FMNL3) to the Arp2/3 complex, driving filopodia formation and membrane protrusion. This pathway??CDC42 ?? FNBP1L ?? N-WASP ?? Arp2/3??controls cell migration, endocytosis, and synaptic plasticity. FNBP1L also interacts with SH3 domain-containing proteins, integrating multiple inputs to regulate actin dynamics.
In Raji B cells, actin remodeling is critical for immune synapse assembly, antigen internalization, and migration. Knockout of FNBP1L in this context allows researchers to dissect how loss of this scaffold affects B-lymphocyte functions and lymphoma cell invasiveness. Because Raji cells are widely used in cancer and immunology, this model provides a direct system to study the role of FNBP1L in B-cell malignancies and to evaluate its contribution to processes such as tumor cell dissemination and immune evasion.
The polyclonal knockout population supports diverse applications: Western blotting to confirm FNBP1L depletion, immunofluorescence for actin and filopodia analysis, transwell migration and invasion assays, and endocytosis assays using fluorescent ligands. Co-immunoprecipitation can probe altered protein interactions with N-WASP or formins. These cells are also useful as a negative control in CDC42 signaling studies or for compound screening. For further information, contact Ascent Research.
