The FXYD5 Knockout Raji Polyclonal Cells product comprises a CRISPR/Cas9-edited polyclonal population of Raji B lymphocytes with targeted disruption of the FXYD5 gene. This heterogeneous knockout model enables loss-of-function analysis of FXYD5, also known as dysadherin, a regulatory subunit of the Na,K-ATPase ion pump. The polyclonal format provides a robust and versatile tool for studying gene function without clonal selection artifacts, making it suitable for a range of biochemical and cell-based assays.
The Raji cell line is an Epstein-Barr virus (EBV)-positive B lymphocyte model derived from a Burkitt lymphoma patient. It is widely employed in immunology and cancer research due to its well-characterized signaling properties and ease of culture. Raji cells exhibit constitutive NF-??B activation and serve as a relevant system for investigating B cell lymphomagenesis, viral oncogenesis, and tumor microenvironment interactions. The EBV-positive background provides a unique context for studying host-virus interplay and lymphoproliferative disorders.
FXYD5 functions as an auxiliary subunit of the Na,K-ATPase, modulating pump kinetics and influencing cell adhesion and migration. It is transcriptionally activated by TGF-?? and NF-??B, with SNAI1, TWIST1, and ??-catenin/TCF acting downstream. FXYD5 promotes epithelial-mesenchymal transition (EMT) by downregulating E-cadherin (CDH1) and upregulating N-cadherin (CDH2), vimentin (VIM), MMP2, and MMP9. Through interaction with ATP1A1 and ATP1B1, FXYD5 regulates ion homeostasis and enhances FAK/SRC-mediated focal adhesion dynamics, driving migration and invasion.
In the Raji B lymphocyte context, knockout of FXYD5 provides a powerful model to dissect its contributions to lymphoma biology and metastasis-associated processes. Given the EBV-positive nature of Raji cells, this system allows exploration of how viral latency and oncogenic signaling converge with FXYD5-mediated pathways. Disruption of FXYD5 may alter Na,K-ATPase activity and EMT-related gene expression, potentially affecting cell proliferation, apoptosis, and chemotaxis. Researchers can leverage this model to investigate the crosstalk between TGF-??, NF-??B, and Wnt/??-catenin pathways in B-cell malignancies.
Research applications include functional dissection of FXYD5 in B-cell lymphoma progression, EMT regulation, and Na,K-ATPase modulation. The polyclonal knockout cells are ideal for high-throughput drug screening against metastasis inhibitors and for analyzing oncogenic signal transduction using techniques such as Western blotting, RT-qPCR, immunofluorescence, flow cytometry, migration and invasion assays, Na,K-ATPase activity measurements, RNA-seq, and co-immunoprecipitation. For detailed technical specifications and ordering information, please contact Ascent Research.
