The GCA Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji B lymphocyte line. This product features targeted disruption of the GCA gene, generating a heterogeneous loss-of-function model for studying grancalcin biology. The polyclonal nature avoids clonal artifacts and provides a representative knockout background.
Raji cells are an immortalized human B lymphocyte line from Burkitt lymphoma, growing in suspension and widely used for B cell signaling, apoptosis, and lymphoma research. The cells express key B cell markers and respond to BCR activation and calcium-mobilizing agents, offering a physiologically relevant host for GCA knockout studies.
Grancalcin (GCA) is a penta-EF-hand calcium-binding protein that, upon calcium binding, translocates to membranes and interacts with S100A12 and L-plastin (LCP1). This complex regulates actin cytoskeleton remodeling and exocytosis by modulating SNARE-mediated vesicle fusion. Upstream, GCA is activated by calcium influx, B cell receptor stimulation, and pharmacological agents like phorbol esters and calcium ionophores. Downstream targets include S100A12, L-plastin, and components of the exocytosis machinery. Representative pathway components??Ca2?, GCA, S100A12, LCP1, SNARE proteins, and actin??place grancalcin at the intersection of calcium signaling and membrane trafficking.
In B lymphocytes, GCA-mediated calcium-dependent exocytosis and cytoskeletal rearrangements are critical for cytokine release, cell adhesion, and migration. GCA knockout in Raji cells is expected to impair secretory responses and adhesion, potentially disrupting B cell activation, immunological synapse formation, and tissue homing. As a Burkitt lymphoma-derived line, this model aids in dissecting grancalcin’s role in lymphomagenesis and drug sensitivity.
Researchers can confirm GCA disruption via Western blotting, RT-qPCR, and Sanger sequencing. Functional assays include calcium flux analysis, cell adhesion and migration assays, and flow cytometry for activation markers or apoptosis. Co-immunoprecipitation and phospho-signaling profiling enable interaction and pathway studies. Applications span B-cell lymphoma biology, inflammation, and autoimmunity, with utility in drug response testing on calcium-dependent pathways. For further technical details or to discuss customized applications, please contact Ascent Research.
