GOLIM4 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population derived from the Jurkat human T lymphocyte line. The GOLIM4 gene has been disrupted via CRISPR/Cas9, abolishing functional protein expression and creating a heterogeneous pool free from clonal selection biases. This live cell product is provided at low passage, ready for expansion and immediate use in functional assays targeting Golgi membrane biology and retrograde transport.
Jurkat cells are an immortalized T lymphoblastoid line from an acute T cell leukemia patient, widely serving as a model for TCR signaling and apoptosis. They express key T cell receptors and signal through components such as ZAP70 and PLC??1 upon engagement. Their robust culture and genetic tractability make them ideal for gene editing. Expressing GOLIM4 knockout in this background allows investigation of Golgi-related processes within a T cell context.
GOLIM4 is a cis-Golgi transmembrane protein that cycles between the Golgi and endosomes, acting as a receptor for the Shiga toxin B subunit to mediate toxin transport to the ER. It interacts with the GARP complex and GOLGA2, and its trafficking is regulated by Golgi pH and retrograde sorting signals. The protein operates within a network of RAB6A, SNAREs, and GRASPs that govern retrograde transport and Golgi structure. Disruption of GOLIM4 allows dissection of how this machinery controls toxin susceptibility and Golgi homeostasis.
In Jurkat T lymphocytes, GOLIM4 knockout provides a powerful tool to study retrograde trafficking and its impact on cellular functions such as receptor surface expression and cytokine secretion. The model is particularly valuable for Shiga toxin research, enabling examination of toxin internalization and ER targeting steps in a human immune cell line. It bridges Golgi biology with T cell physiology, offering insights into toxin-induced pathogenesis like hemolytic uremic syndrome.
Researchers can employ these cells for immunofluorescence to visualize Golgi morphology, Shiga toxin internalization assays using fluorescent toxin subunits, Western blotting, RT-qPCR, and flow cytometry. The polyclonal pool supports population-level studies and is suitable for genetic screens and drug testing. For inquiries or purchasing, please contact Ascent Research.
