The GORASP1 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed for disruption of the GORASP1 gene in the Jurkat T lymphoblast cell line. This polyclonal format provides a heterogeneous pool of knockout cells, avoiding clonal bias and enabling robust functional studies. The product is an ideal tool for investigating Golgi biology, cell migration, and T-cell signaling.
The Jurkat parental line is an immortalized T lymphocyte model derived from a 14-year-old male with acute T-cell leukemia. Widely used for studying T-cell receptor signaling, cytokine production, and apoptosis, these cells also serve as a model for T-cell malignancies. The combination with GORASP1 disruption allows exploration of Golgi-dependent processes in a leukemic T-cell context.
GORASP1 encodes a Golgi matrix protein critical for cisternal stacking and ribbon formation. Its activity is regulated by CDK1 and ERK-mediated phosphorylation, which drives mitotic Golgi disassembly, while dephosphorylation permits reassembly. GORASP1 interacts with GM130 (GOLGA2), GRASP55 (GORASP2), and p115 (USO1) within the COPI complex. Beyond architecture, it facilitates unconventional secretion and regulates cell adhesion/migration via Golgi reorganization. This signaling network positions GORASP1 at the intersection of cell cycle control and secretory trafficking.
In Jurkat cells, GORASP1 disruption provides a system to examine Golgi dynamics in immune cell function. T-cell activation requires polarized secretion of cytokines, a process dependent on intact Golgi ribbon integrity. Loss of GORASP1 may impair this, affecting immune responses. Additionally, the leukemia background enables study of Golgi fragmentation in cancer cell migration and invasion. This knockout model thus supports research into both T-cell biology and leukemic pathophysiology.
Key applications include immunofluorescence microscopy to visualize Golgi morphology, flow cytometry for cell cycle analysis, transwell migration/invasion assays, and western blotting with phospho-specific antibodies to assess knockout impact. RT-qPCR confirms GORASP1 disruption. This polyclonal population is also suitable for unconventional secretion studies and compound screening targeting Golgi-dependent pathways in leukemia. For further information or custom requests, please contact Ascent Research.
