GPALPP1 Knockout HEK293T Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Kidney

Datasheet

Research applications for the GPALPP1 Knockout HEK293T Polyclonal Cells span functional genomics, lipid biology, and drug discovery. Investigators can employ this model for detailed mechanistic studies using lipid uptake assays, lipoprotein binding experiments, and co-immunoprecipitation to map protein interactions among GPALPP1, GPIHBP1, LPL, and apolipoproteins. Activation of lipase activity can be monitored via enzymatic assays, […]

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Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

HEK293T

Sex of Donor

Female

Age

Fetus

Derived From Site

Fetal kidney

Gene Name

GPALPP1

Gene Identifier

NCBI Gene ID 55425

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

DMEM

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

Research applications for the GPALPP1 Knockout HEK293T Polyclonal Cells span functional genomics, lipid biology, and drug discovery.

Investigators can employ this model for detailed mechanistic studies using lipid uptake assays, lipoprotein binding experiments, and co-immunoprecipitation to map protein interactions among GPALPP1, GPIHBP1, LPL, and apolipoproteins.

Activation of lipase activity can be monitored via enzymatic assays, while expression changes in related pathway components can be quantified through RT-qPCR and Western blotting.

Immunofluorescence and flow cytometry enable assessment of protein localization and surface abundance.

These tools facilitate target validation for atherosclerosis, obesity, and other metabolic diseases, as well as exploration of GPI-anchored protein trafficking. The polyclonal knockout cells serve as a versatile resource for hypothesis-driven research and preclinical development. For further information or technical assistance, please contact Ascent Research.