The GPALPP1 Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population that disrupts the human GPALPP1 gene in the cervical adenocarcinoma-derived HeLa cell line. This loss-of-function model captures the genetic diversity of a pooled knockout population, avoiding clonal selection artifacts and enabling robust functional genomic analyses of GPALPP1-dependent signaling.
HeLa cells are an immortalized epithelial line originating from a cervical adenocarcinoma, harboring integrated HPV18 sequences that express E6 and E7 oncoproteins. These viral proteins inactivate p53 and retinoblastoma (pRb) tumor suppressors, creating a permissive environment for oncogenic transformation and establishing HeLa as a foundational model for cancer biology, HPV pathogenesis, and molecular signaling research.
The GPALPP1 gene encodes a putative GPCR-associated adaptor protein that interacts with GPCR complexes, G proteins, ??-arrestins, and scaffolding proteins to modulate intracellular signaling. It is implicated in transmitting signals downstream to the MAPK/ERK pathway through EGFR, Ras, Raf, MEK, and ERK1/2, as well as to PI3K-AKT and cAMP?CPKA?CCREB cascades. Upstream regulators include GPCR ligands, EGFR, and AP-1 transcription factors, while GPALPP1 activity impacts downstream effectors such as ERK1/2, AKT, Rho GTPases, and the F-actin cytoskeleton.
In the HeLa context, HPV E6/E7-driven inactivation of p53 and pRb intersects with multiple signaling nodes potentially coordinated by GPALPP1, including proliferative and migratory pathways. Disruption of GPALPP1 allows dissection of its contribution to HPV-mediated oncogenesis and evaluation of how loss of this adaptor alters the balance of MAPK/ERK and PI3K-AKT activities. The polyclonal knockout format thus provides a powerful tool for investigating GPCR-associated signaling dependencies in cervical cancer research.
This product is suitable for GPCR signaling studies, cancer cell signaling research, HPV oncogenesis investigations, drug target validation, and functional genomics. Representative assays include western blot and RT-qPCR for expression analysis, immunofluorescence for localization of signaling components like F-actin, cell proliferation and migration/invasion assays, phospho-signaling analysis (phospho-ERK, phospho-AKT), GPCR functional assays measuring cAMP, and apoptosis assays. For technical inquiries, please contact Ascent Research.
